BRENDA - Enzyme Database show

Carnosine-anserine synthetase of muscle. 4. Partial purification of the enzyme and further studies of beta-alanyl peptide synthesis

Stenesh, J.J.; Winnick, T.; Biochem. J. 77, 575-581 (1960)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
6.3.2.11
most of the activity is lost upon freezing or upon dialysis with EDTA-treated cellophan tubing for several h at 0°C
Gallus gallus
6.3.2.11
pH 7.0, 25°C, 1 h, with crystalline ribonuclease, not significantly inactivated
Gallus gallus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.2.11
diphosphate
-
Gallus gallus
6.3.2.11
F-
-
Gallus gallus
6.3.2.11
Fe2+
weak
Gallus gallus
6.3.2.11
K+
10-100 mM, weak inhibition
Gallus gallus
6.3.2.11
Mo2+
weak
Gallus gallus
6.3.2.11
Ni2+
-
Gallus gallus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.2.11
Mg2+
maximal activity at 2 mM, inhibition at higher concentrations
Gallus gallus
6.3.2.11
Mn2+
can substitute for Mg2+, more effective at low concentration
Gallus gallus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.2.11
Gallus gallus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.2.11
partial
Gallus gallus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.2.11
muscle
pectoral
Gallus gallus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.2.11
ATP + L-His + beta-Ala
not r
1000
Gallus gallus
ADP + phosphate + carnosine
-
1000
Gallus gallus
-
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
6.3.2.11
6
8
stable in the range
Gallus gallus
General Stability (protein specific)
EC Number
General Stability
Organism
6.3.2.11
most of the activity is lost upon freezing or upon dialysis with EDTA-treated cellophan tubing for several h at 0°C
Gallus gallus
6.3.2.11
pH 7.0, 25°C, 1 h, with crystalline ribonuclease, not significantly inactivated
Gallus gallus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.2.11
diphosphate
-
Gallus gallus
6.3.2.11
F-
-
Gallus gallus
6.3.2.11
Fe2+
weak
Gallus gallus
6.3.2.11
K+
10-100 mM, weak inhibition
Gallus gallus
6.3.2.11
Mo2+
weak
Gallus gallus
6.3.2.11
Ni2+
-
Gallus gallus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.2.11
Mg2+
maximal activity at 2 mM, inhibition at higher concentrations
Gallus gallus
6.3.2.11
Mn2+
can substitute for Mg2+, more effective at low concentration
Gallus gallus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.2.11
partial
Gallus gallus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.2.11
muscle
pectoral
Gallus gallus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.2.11
ATP + L-His + beta-Ala
not r
1000
Gallus gallus
ADP + phosphate + carnosine
-
1000
Gallus gallus
-
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
6.3.2.11
6
8
stable in the range
Gallus gallus