Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bulmer, G.; de Andrade, P.; Field, R.; van Munster, J.
    Recent advances in enzymatic synthesis of beta-glucan and cellulose (2021), Carbohydr. Res., 508, 108411.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.30 recombinant enzyme expression in Escherichia coli Euglena gracilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.30 enzyme crystal structure in the absence of substrate (PDB ID 6HQ6) and in complex with laminarihexaose (PDB ID 6HQ8) uncultured bacterium
2.4.1.31 enzyme crystal structure analysis, PDB ID 6GH2 Paenibacillus sp. YM1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate Euglena gracilis
-
 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate Ochromonas sp.
-
 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate Paenibacillus polymyxa
-
 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.30 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate Euglena gracilis
-
 [(1->3)-beta-D-glucosyl]n + phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate Ochromonas sp.
-
 [(1->3)-beta-D-glucosyl]n + phosphate
-
 r
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate Paenibacillus sp. YM1
-
 D-glucose + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.30 Euglena gracilis A0A8D4WWT7
-
-
2.4.1.30 Ochromonas sp.
-
-
-
2.4.1.30 Paenibacillus polymyxa
-
-
-
2.4.1.30 uncultured bacterium
-
-
-
2.4.1.31 Paenibacillus sp. YM1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.30 additional information crystalline oligosaccharides (DP 30) are produced by a partially purified beta-1,3-D-glucan phosphorylase extract from the microalga Euglena gracilis Euglena gracilis ?
-
-
2.4.1.30 additional information recombinant enzyme PapP shows neither synthetic activity in the presence of D-Glc and alpha-D-Glc-1P, nor phosphorolysis activity towards Glc-Glc disaccharides with different linkages. Nonetheless, phosphorolysis assays with DP3 to DP6 reveal its preference for longer substrates. These results strongly suggest that PapP can only operate on beta-1,3 linear oligosaccharide acceptors with DP greater than 2, evidencing its different specificity from GH149 glucan phosphorylases Paenibacillus polymyxa ?
-
-
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Euglena gracilis [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Ochromonas sp. [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Paenibacillus polymyxa [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.30 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 Euglena gracilis [(1->3)-beta-D-glucosyl]n + phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 Ochromonas sp. [(1->3)-beta-D-glucosyl]n + phosphate
-
 r
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate
-
 Paenibacillus sp. YM1 D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 D-glucose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-D-glucose + phosphate
-
 r
2.4.1.31 D-glucose + alpha-D-mannose 1-phosphate very low activity Paenibacillus sp. YM1 beta-D-mannopyranosyl-1,3-D-glucose + phosphate
-
 ?
2.4.1.31 additional information the enzyme shows a broader substrate specificty, with specificity for disaccharides. It also catalyses the synthesis of beta-D-mannopyranosyl-1,3-D-glucopyranose from D-Glc and alpha-D-Man-1P, albeit with highly reduced catalytic efficiency. The loss of an important hydrogen bond between the axial hydroxy group at C2 and a key residue in the active site, thus possibly contributing to the low reaction turnover Paenibacillus sp. YM1 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.30 algal beta-1,3-glucan phosphorylase
-
 Euglena gracilis
2.4.1.30 algal beta-1,3-glucan phosphorylase
-
 Ochromonas sp.
2.4.1.30 beta-1,3-D-glucan phosphorylase
-
 Euglena gracilis
2.4.1.30 beta-1,3-D-glucan phosphorylase
-
 Ochromonas sp.
2.4.1.30 beta-1,3-D-glucan phosphorylase
-
 Paenibacillus polymyxa
2.4.1.30 beta-1,3-D-glucan phosphorylase
-
 uncultured bacterium
2.4.1.30 EgP1
-
 Euglena gracilis
2.4.1.30 OcP1
-
 Ochromonas sp.
2.4.1.30 PapP
-
 Paenibacillus polymyxa
2.4.1.30 Pro_7066
-
 uncultured bacterium
2.4.1.31 PsLBP
-
 Paenibacillus sp. YM1

General Information

EC Number General Information Comment Organism
2.4.1.30 evolution the enzyme belongs to glycoside family 149, GH149 Euglena gracilis
2.4.1.30 evolution the enzyme belongs to glycoside family 161, GH161 Ochromonas sp.
2.4.1.30 evolution the enzyme belongs to glycoside family 161, GH161 Paenibacillus polymyxa
2.4.1.30 evolution the enzyme belongs to glycoside family 149, GH149. Although the overall domain organisation is similar to GH94, Pro_7066 enzyme contains two additional distinct domains flanking its catalytic region and a surface oligosaccharide binding site where laminarihexaose is accommodated, which is distant from the catalytic site and may be involved in the recognition of longer substrates uncultured bacterium
2.4.1.31 evolution enzyme LBP belongs to the glycoside family 94, GH94 Paenibacillus sp. YM1