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Literature summary extracted from

  • Ubiparip, Z.; De Doncker, M.; Beerens, K.; Franceus, J.; Desmet, T.
    beta-Glucan phosphorylases in carbohydrate synthesis (2021), Appl. Microbiol. Biotechnol., 105, 4073-4087.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.30 synthesis beta-1,3-glucans draw considerable attention for their proven beneficial effects on immunomodulation, cholesterol levels, and glycemic control, and their use as additives in food or moisturizing personal care products. Usage of the enzyme for beta-1,3-glucan synthesis Ochromonas danica
2.4.1.30 synthesis beta-1,3-glucans draw considerable attention for their proven beneficial effects on immunomodulation, cholesterol levels, and glycemic control, and their use as additives in food or moisturizing personal care products. Usage of the enzyme for beta-1,3-glucan synthesis Poterioochromonas malhamensis
2.4.1.30 synthesis beta-1,3-glucans draw considerable attention for their proven beneficial effects on immunomodulation, cholesterol levels, and glycemic control, and their use as additives in food or moisturizing personal care products. Usage of the enzyme for beta-1,3-glucan synthesis Euglena gracilis
2.4.1.97 synthesis beta-1,3-glucans draw considerable attention for their proven beneficial effects on immunomodulation, cholesterol levels, and glycemic control, and their use as additives in food or moisturizing personal care products. Usage of the enzyme for beta-1,3-glucan synthesis Thermosipho africanus
2.4.1.97 synthesis beta-1,3-glucans draw considerable attention for their proven beneficial effects on immunomodulation, cholesterol levels, and glycemic control, and their use as additives in food or moisturizing personal care products. Usage of the enzyme for beta-1,3-glucan synthesis Paenibacillus polymyxa
2.4.1.97 synthesis beta-1,3-glucans draw considerable attention for their proven beneficial effects on immunomodulation, cholesterol levels, and glycemic control, and their use as additives in food or moisturizing personal care products. Usage of the enzyme for beta-1,3-glucan synthesis Ochromonas danica

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.30 additional information the inability of some beta-1,3-oligoglucan phosphorylases (BOPs) and beta-1,3-polyglucan (or laminarin) phosphorylases (BGPs, EC 2.4.1.97) to use glucose as an acceptor for beta-glucan synthesis can be overcome through coupled reactions with laminaribiose phosphorylases (EC 2.4.1.31) Ochromonas danica
2.4.1.30 additional information the inability of some beta-1,3-oligoglucan phosphorylases (BOPs) and beta-1,3-polyglucan (or laminarin) phosphorylases (BGPs, EC 2.4.1.97) to use glucose as an acceptor for beta-glucan synthesis can be overcome through coupled reactions with laminaribiose phosphorylases (EC 2.4.1.31) Poterioochromonas malhamensis
2.4.1.30 additional information the inability of some beta-1,3-oligoglucan phosphorylases (BOPs) and beta-1,3-polyglucan (or laminarin) phosphorylases (BGPs, EC 2.4.1.97) to use glucose as an acceptor for beta-glucan synthesis can be overcome through coupled reactions with laminaribiose phosphorylases (EC 2.4.1.31) Euglena gracilis
2.4.1.31 additional information the inability of some beta-1,3-oligoglucan phosphorylases (BOPs, EC 2.4.1.30) and beta-1,3-polyglucan (or laminarin) phosphorylases (BGPs, EC 2.4.1.97) to use glucose as an acceptor for beta-glucan synthesis can be overcome through coupled reactions with laminaribiose phosphorylases Acholeplasma laidlawii
2.4.1.31 additional information the inability of some beta-1,3-oligoglucan phosphorylases (BOPs, EC 2.4.1.30) and beta-1,3-polyglucan (or laminarin) phosphorylases (BGPs, EC 2.4.1.97) to use glucose as an acceptor for beta-glucan synthesis can be overcome through coupled reactions with laminaribiose phosphorylases Paenibacillus sp. YM1
2.4.1.97 additional information the inability of some beta-1,3-oligoglucan phosphorylases (BOPs, EC 2.4.1.30) and beta-1,3-polyglucan (or laminarin) phosphorylases (BGPs) to use glucose as an acceptor for beta-glucan synthesis can be overcome through coupled reactions with laminaribiose phosphorylases (EC 2.4.1.31) Thermosipho africanus
2.4.1.97 additional information the inability of some beta-1,3-oligoglucan phosphorylases (BOPs, EC 2.4.1.30) and beta-1,3-polyglucan (or laminarin) phosphorylases (BGPs) to use glucose as an acceptor for beta-glucan synthesis can be overcome through coupled reactions with laminaribiose phosphorylases (EC 2.4.1.31) Paenibacillus polymyxa
2.4.1.97 additional information the inability of some beta-1,3-oligoglucan phosphorylases (BOPs, EC 2.4.1.30) and beta-1,3-polyglucan (or laminarin) phosphorylases (BGPs) to use glucose as an acceptor for beta-glucan synthesis can be overcome through coupled reactions with laminaribiose phosphorylases (EC 2.4.1.31) Ochromonas danica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.30 0.6
-
 D-glucose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.30 0.7
-
 laminaribiose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.30 1.3
-
 laminaritriose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.30 1.4
-
 laminaritetraose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.30 2.3
-
 Laminaripentaose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.30 2.9
-
 Laminarihexaose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.31 0.4
-
 D-glucose pH 6.0, 40°C Acholeplasma laidlawii
2.4.1.31 0.7
-
 2-deoxy-D-glucose pH 6.0, 40°C Acholeplasma laidlawii
2.4.1.31 6
-
 D-glucose pH 6.8-7.0, 55°C Paenibacillus sp. YM1
2.4.1.97 1
-
 laminaritriose pH and temperature not specified in the publication Paenibacillus polymyxa
2.4.1.97 1.6
-
 alpha-D-glucose 1-phosphate pH 7.5, 75°C Thermosipho africanus
2.4.1.97 1.6
-
 laminaribiose pH and temperature not specified in the publication Paenibacillus polymyxa
2.4.1.97 1.6
-
 Laminaripentaose pH and temperature not specified in the publication Paenibacillus polymyxa
2.4.1.97 1.8
-
 laminaritetraose pH and temperature not specified in the publication Paenibacillus polymyxa
2.4.1.97 2.3
-
 Laminarihexaose pH and temperature not specified in the publication Paenibacillus polymyxa
2.4.1.97 2.6
-
 cellopentaose pH 7.5, 75°C Thermosipho africanus
2.4.1.97 3.7
-
 cellotetraose pH 7.5, 75°C Thermosipho africanus
2.4.1.97 4
-
 cellotriose pH 7.5, 75°C Thermosipho africanus
2.4.1.97 7.5
-
 cellobiose pH 7.5, 75°C Thermosipho africanus
2.4.1.97 14.3
-
 D-glucose pH 7.5, 75°C Thermosipho africanus
2.4.1.97 25.7
-
 xylose pH 7.5, 75°C Thermosipho africanus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate Ochromonas danica
-
 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate Poterioochromonas malhamensis
-
 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate Euglena gracilis
-
 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate Acholeplasma laidlawii
-
 D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate Paenibacillus sp. YM1
-
 D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate Acholeplasma laidlawii PG-8A
-
 D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate Thermosipho africanus
-
 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate Paenibacillus polymyxa
-
 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate Ochromonas danica
-
 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate Thermosipho africanus TCF52B
-
 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate Paenibacillus polymyxa ATCC 842
-
 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.30 Euglena gracilis A0A8D4WWT7
-
-
2.4.1.30 Ochromonas danica
-
-
-
2.4.1.30 Poterioochromonas malhamensis
-
-
-
2.4.1.31 Acholeplasma laidlawii
-
-
-
2.4.1.31 Acholeplasma laidlawii PG-8A
-
-
-
2.4.1.31 Paenibacillus sp. YM1
-
-
-
2.4.1.97 Ochromonas danica A0A146IWE2
-
-
2.4.1.97 Paenibacillus polymyxa A0A143T443 Bacillus polymyxa
-
2.4.1.97 Paenibacillus polymyxa ATCC 842 A0A143T443 Bacillus polymyxa
-
2.4.1.97 Thermosipho africanus
-
-
-
2.4.1.97 Thermosipho africanus TCF52B
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.30 2-deoxy-D-glucose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 6-deoxy-D-glucose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 alpha-methylglucoside + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 beta-methylglucoside + alpha-D-glucose 1-phosphate
-
 Poterioochromonas malhamensis ? + phosphate
-
 r
2.4.1.30 beta-methylglucoside + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 cellobiose + alpha-D-glucose 1-phosphate
-
 Poterioochromonas malhamensis cellotriose + phosphate
-
 r
2.4.1.30 cellobiose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis cellotriose + phosphate
-
 r
2.4.1.30 cellopentaose + alpha-D-glucose 1-phosphate
-
 Ochromonas danica cellohexaose + phosphate
-
 r
2.4.1.30 cellopentaose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis cellohexaose + phosphate
-
 r
2.4.1.30 cellotetraose + alpha-D-glucose 1-phosphate
-
 Ochromonas danica cellopentaose + phosphate
-
 r
2.4.1.30 cellotetraose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis cellopentaose + phosphate
-
 r
2.4.1.30 cellotriose + alpha-D-glucose 1-phosphate
-
 Ochromonas danica cellotetraose + phosphate
-
 r
2.4.1.30 cellotriose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis cellotetraose + phosphate
-
 r
2.4.1.30 chrysolaminarin + alpha-D-glucose 1-phosphate
-
 Poterioochromonas malhamensis ? + phosphate
-
 r
2.4.1.30 chrysolaminarin + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 D-glucose + alpha-D-glucose 1-phosphate
-
 Poterioochromonas malhamensis laminaribiose + phosphate
-
 r
2.4.1.30 D-glucose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis laminaribiose + phosphate
-
 r
2.4.1.30 D-maltose + alpha-D-glucose 1-phosphate
-
 Poterioochromonas malhamensis maltotriose + phosphate
-
 r
2.4.1.30 D-maltose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis maltotriose + phosphate
-
 r
2.4.1.30 fructose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 gentiobiose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 L-arabinose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 L-idose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 L-sorbose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 laminaribiose + alpha-D-glucose 1-phosphate
-
 Ochromonas danica laminaritriose + phosphate
-
 r
2.4.1.30 laminaribiose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis laminaritriose + phosphate
-
 r
2.4.1.30 laminarihexaose + alpha-D-glucose 1-phosphate
-
 Ochromonas danica laminariheptaose + phosphate
-
 r
2.4.1.30 laminarihexaose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis laminariheptaose + phosphate
-
 r
2.4.1.30 laminarin + alpha-D-glucose 1-phosphate
-
 Poterioochromonas malhamensis ? + phosphate
-
 r
2.4.1.30 laminarin + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 laminaripentaose + alpha-D-glucose 1-phosphate
-
 Ochromonas danica laminarihexaose + phosphate
-
 r
2.4.1.30 laminaripentaose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis laminarihexaose + phosphate
-
 r
2.4.1.30 laminaritetraose + alpha-D-glucose 1-phosphate
-
 Ochromonas danica laminaripentaose + phosphate
-
 r
2.4.1.30 laminaritetraose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis laminaripentaose + phosphate
-
 r
2.4.1.30 laminaritriose + alpha-D-glucose 1-phosphate
-
 Ochromonas danica laminaritetraose + phosphate
-
 r
2.4.1.30 laminaritriose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis laminaritetraose + phosphate
-
 r
2.4.1.30 additional information the enzyme can synthesize carbohydrates of DP 2-30 with beta-1,3 linkages. Substrates are laminaribiose (Lam2), Lam3, Lam4, Lam5, Lam6, laminarin, cellotriose (Cel3), Cel4, Cel5, sophorose, methyl beta-D-glucopyranoside, and p-nitrophenyl beta-D-glucopyranoside Ochromonas danica ?
-
-
2.4.1.30 additional information the enzyme can utilize the substrates laminarin, chrysolaminarin, cellobiose, glucose, maltose, and beta-methylglucoside Poterioochromonas malhamensis ?
-
-
2.4.1.30 additional information the enzyme has a broad acceptor specificity. Substrate are D-glucose, laminaribiose (Lam2), Lam3, Lam4, Lam5, and Lam6, as well as laminarin, allose, mannose, galactose, L-idose, beta-methyl-D-glucoside, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, glucosamine, N-acetylglucosamine, D- or L-xylose, sophorose, cellobiose, gentiobiose, phenyl-beta-D-glucoside, O-nitrophenyl-beta-D-glucoside, m-nitrophenyl-beta-D-glucoside, p-nitrophenyl-beta-D-glucoside, salicin, maltose, paramylon treated with KOH, alpha-methylglucoside, beta-methylglucoside, 6-deoxyglucose, lyxose, ribose, L-arabinose, fructose, L-sorbose, sucrose Euglena gracilis ?
-
-
2.4.1.30 phenyl-beta-D-glucoside + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 salicin + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 sophorose + alpha-D-glucose 1-phosphate
-
 Ochromonas danica ? + phosphate
-
 r
2.4.1.30 sophorose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 sucrose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis ? + phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Ochromonas danica [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Poterioochromonas malhamensis [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Euglena gracilis [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 Ochromonas danica [(1->3)-beta-D-glucosyl]n + phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 Poterioochromonas malhamensis [(1->3)-beta-D-glucosyl]n + phosphate
-
 r
2.4.1.30 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 Euglena gracilis [(1->3)-beta-D-glucosyl]n + phosphate
-
 r
2.4.1.31 1,5-anhydro-D-glucitol + alpha-D-glucose 1-phosphate
-
 Acholeplasma laidlawii 3-beta-D-glucosyl-1,5-anhydro-D-glucitol + phosphate
-
 r
2.4.1.31 2-deoxy-D-glucose + alpha-D-glucose 1-phosphate
-
 Acholeplasma laidlawii 3-beta-D-glucosyl-2-deoxy-D-glucose + phosphate
-
 r
2.4.1.31 2-deoxy-D-glucose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-2-deoxy-D-glucose + phosphate
-
 r
2.4.1.31 2-deoxy-D-glucose + alpha-D-glucose 1-phosphate
-
 Acholeplasma laidlawii PG-8A 3-beta-D-glucosyl-2-deoxy-D-glucose + phosphate
-
 r
2.4.1.31 3-beta-D-glucosyl-D-glucose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 laminaritriose + phosphate
-
 r
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate
-
 Acholeplasma laidlawii D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate
-
 Paenibacillus sp. YM1 D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate
-
 Acholeplasma laidlawii PG-8A D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 4-deoxy-D-glucose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-4-deoxy-D-glucose + phosphate
-
 r
2.4.1.31 6-deoxy-D-glucose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-6-deoxy-D-glucose + phosphate
-
 r
2.4.1.31 allose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-allose + phosphate
-
 r
2.4.1.31 cellobiose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 cellotriose + phosphate
-
 r
2.4.1.31 D-galactose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-D-galactose + phosphate
-
 r
2.4.1.31 D-glucosamine + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-D-glucosamine + phosphate
-
 r
2.4.1.31 D-glucose + alpha-D-glucose 1-phosphate
-
 Acholeplasma laidlawii 3-beta-D-glucosyl-D-glucose + phosphate
-
 r
2.4.1.31 D-glucose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-D-glucose + phosphate
-
 r
2.4.1.31 D-glucose + alpha-D-glucose 1-phosphate
-
 Acholeplasma laidlawii PG-8A 3-beta-D-glucosyl-D-glucose + phosphate
-
 r
2.4.1.31 D-glucuronic acid + alpha-D-glucose 1-phosphate
-
 Acholeplasma laidlawii 3-beta-D-glucosyl-D-glucuronate + phosphate
-
 r
2.4.1.31 D-glucuronic acid + alpha-D-glucose 1-phosphate
-
 Acholeplasma laidlawii PG-8A 3-beta-D-glucosyl-D-glucuronate + phosphate
-
 r
2.4.1.31 D-lyxose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-D-lyxose + phosphate
-
 r
2.4.1.31 D-xylose + alpha-D-glucose 1-phosphate
-
 Acholeplasma laidlawii 3-beta-D-glucosyl-D-xylose + phosphate
-
 r
2.4.1.31 D-xylose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-D-xylose + phosphate
-
 r
2.4.1.31 D-xylose + alpha-D-glucose 1-phosphate
-
 Acholeplasma laidlawii PG-8A 3-beta-D-glucosyl-D-xylose + phosphate
-
 r
2.4.1.31 L-arabinose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-L-arabinose + phosphate
-
 r
2.4.1.31 L-idose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-L-idose + phosphate
-
 r
2.4.1.31 L-sorbose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-L-sorbose + phosphate
-
 r
2.4.1.31 mannose + alpha-D-glucose 1-phosphate
-
 Acholeplasma laidlawii 3-beta-D-glucosyl-mannose + phosphate
-
 r
2.4.1.31 additional information the enzyme has a broad acceptor specificity. Substrate are D-glucose, mannose, allose, galactose, L-idose, alpha-methylglucoside, beta-methylglucoside, 1,5-anhydroglucitol, 2-deoxy-D-glucose, glucosamine, N-acetylglucosamine, 4-deoxy-D-glucose, 6-deoxy-D-glucose, xylose, lyxose, ribose, L-arabinose, fructose, L-sorbose, phenyl beta-glucoside, cellobiose, laminaribiose, sucrose Paenibacillus sp. YM1 ?
-
-
2.4.1.31 N-acetyl-D-glucosamine + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-N-acetyl-D-glucosamine + phosphate
-
 r
2.4.1.31 sucrose + alpha-D-glucose 1-phosphate
-
 Paenibacillus sp. YM1 3-beta-D-glucosyl-sucrose + phosphate
-
 r
2.4.1.97 cellobiose + alpha-D-glucose 1-phosphate
-
 Thermosipho africanus cellotriose + phosphate
-
 r
2.4.1.97 cellobiose + alpha-D-glucose 1-phosphate
-
 Thermosipho africanus TCF52B cellotriose + phosphate
-
 r
2.4.1.97 cellopentaose + alpha-D-glucose 1-phosphate
-
 Thermosipho africanus cellohexaose + phosphate
-
 r
2.4.1.97 cellotetraose + alpha-D-glucose 1-phosphate
-
 Thermosipho africanus cellopentaose + phosphate
-
 r
2.4.1.97 cellotriose + alpha-D-glucose 1-phosphate
-
 Thermosipho africanus cellotetraose + phosphate
-
 r
2.4.1.97 D-glucose + alpha-D-glucose 1-phosphate
-
 Thermosipho africanus laminaribiose + phosphate
-
 r
2.4.1.97 D-glucose + alpha-D-glucose 1-phosphate
-
 Thermosipho africanus TCF52B laminaribiose + phosphate
-
 r
2.4.1.97 laminaribiose + alpha-D-glucose 1-phosphate
-
 Paenibacillus polymyxa laminaritriose + phosphate
-
 r
2.4.1.97 laminaribiose + alpha-D-glucose 1-phosphate
-
 Paenibacillus polymyxa ATCC 842 laminaritriose + phosphate
-
 r
2.4.1.97 laminarihexaose + alpha-D-glucose 1-phosphate
-
 Paenibacillus polymyxa laminariheptaose + phosphate
-
 r
2.4.1.97 laminaripentaose + alpha-D-glucose 1-phosphate
-
 Paenibacillus polymyxa laminarihexaose + phosphate
-
 r
2.4.1.97 laminaritetraose + alpha-D-glucose 1-phosphate
-
 Paenibacillus polymyxa laminaripentaose + phosphate
-
 r
2.4.1.97 laminaritriose + alpha-D-glucose 1-phosphate
-
 Paenibacillus polymyxa laminaritetraose + phosphate
-
 r
2.4.1.97 additional information beta-glucan phosphorylases (beta-GPs) are carbohydrate-active enzymes that catalyze the degradation of beta-glucans (beta-Gs) with the use of inorganic phosphate, yielding alpha-D-glucose 1-phosphate (alpha-G1P) and a shorter carbohydrate chain as products. Because of the high energy content of the glucosyl phosphate, the reaction is readily reversible and can be used for the synthetic purposes of beta-glucans in vitro with a degree of polymerization (DP) of 3-34 and (1->3)-linkages Thermosipho africanus ?
-
-
2.4.1.97 additional information beta-glucan phosphorylases (beta-GPs) are carbohydrate-active enzymes that catalyze the degradation of beta-glucans (beta-Gs) with the use of inorganic phosphate, yielding alpha-D-glucose 1-phosphate (alpha-G1P) and a shorter carbohydrate chain as products. Because of the high energy content of the glucosyl phosphate, the reaction is readily reversible and can be used for the synthetic purposes of beta-glucans in vitro with a degree of polymerization (DP) of 3-34 and (1->3)-linkages Paenibacillus polymyxa ?
-
-
2.4.1.97 additional information beta-glucan phosphorylases (beta-GPs) are carbohydrate-active enzymes that catalyze the degradation of beta-glucans (beta-Gs) with the use of inorganic phosphate, yielding alpha-D-glucose 1-phosphate (alpha-G1P) and a shorter carbohydrate chain as products. Because of the high energy content of the glucosyl phosphate, the reaction is readily reversible and can be used for the synthetic purposes of beta-glucans in vitro with a degree of polymerization (DP) of 3-34 and (1->3)-linkages Ochromonas danica ?
-
-
2.4.1.97 additional information beta-glucan phosphorylases (beta-GPs) are carbohydrate-active enzymes that catalyze the degradation of beta-glucans (beta-Gs) with the use of inorganic phosphate, yielding alpha-D-glucose 1-phosphate (alpha-G1P) and a shorter carbohydrate chain as products. Because of the high energy content of the glucosyl phosphate, the reaction is readily reversible and can be used for the synthetic purposes of beta-glucans in vitro with a degree of polymerization (DP) of 3-34 and (1->3)-linkages Paenibacillus polymyxa ATCC 842 ?
-
-
2.4.1.97 xylose + alpha-D-glucose 1-phosphate
-
 Thermosipho africanus ? + phosphate
-
 r
2.4.1.97 [(1->3)-beta-D-glucosyl]n + alpha-D-glucose 1-phosphate
-
 Thermosipho africanus [(1->3)-beta-D-glucosyl]n+1 + phosphate
-
 r
2.4.1.97 [(1->3)-beta-D-glucosyl]n + alpha-D-glucose 1-phosphate
-
 Paenibacillus polymyxa [(1->3)-beta-D-glucosyl]n+1 + phosphate
-
 r
2.4.1.97 [(1->3)-beta-D-glucosyl]n + alpha-D-glucose 1-phosphate
-
 Ochromonas danica [(1->3)-beta-D-glucosyl]n+1 + phosphate
-
 r
2.4.1.97 [(1->3)-beta-D-glucosyl]n + alpha-D-glucose 1-phosphate
-
 Thermosipho africanus TCF52B [(1->3)-beta-D-glucosyl]n+1 + phosphate
-
 r
2.4.1.97 [(1->3)-beta-D-glucosyl]n + alpha-D-glucose 1-phosphate
-
 Paenibacillus polymyxa ATCC 842 [(1->3)-beta-D-glucosyl]n+1 + phosphate
-
 r
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Thermosipho africanus [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Paenibacillus polymyxa [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Ochromonas danica [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Thermosipho africanus [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Paenibacillus polymyxa [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Ochromonas danica [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Thermosipho africanus TCF52B [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Thermosipho africanus TCF52B [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Paenibacillus polymyxa ATCC 842 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.97 [(1->3)-beta-D-glucosyl]n + phosphate
-
 Paenibacillus polymyxa ATCC 842 [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.30 beta-1,3-oligoglucan phosphorylase
-
 Ochromonas danica
2.4.1.30 beta-1,3-oligoglucan phosphorylase
-
 Poterioochromonas malhamensis
2.4.1.30 beta-1,3-oligoglucan phosphorylase
-
 Euglena gracilis
2.4.1.30 BOP
-
 Ochromonas danica
2.4.1.30 BOP
-
 Poterioochromonas malhamensis
2.4.1.30 BOP
-
 Euglena gracilis
2.4.1.31 LBP
-
 Acholeplasma laidlawii
2.4.1.31 LBP
-
 Paenibacillus sp. YM1
2.4.1.97 beta-1,3-oligoglucan phosphorylase
-
 Thermosipho africanus
2.4.1.97 beta-1,3-oligoglucan phosphorylase
-
 Paenibacillus polymyxa
2.4.1.97 beta-1,3-oligoglucan phosphorylase
-
 Ochromonas danica
2.4.1.97 beta-GP
-
 Thermosipho africanus
2.4.1.97 beta-GP
-
 Paenibacillus polymyxa
2.4.1.97 beta-GP
-
 Ochromonas danica
2.4.1.97 Bgp
-
 Thermosipho africanus
2.4.1.97 Bgp
-
 Paenibacillus polymyxa
2.4.1.97 Bgp
-
 Ochromonas danica
2.4.1.97 laminarin phosphorylase
-
 Thermosipho africanus
2.4.1.97 laminarin phosphorylase
-
 Paenibacillus polymyxa
2.4.1.97 OdBGP
-
 Ochromonas danica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.30 22.5
-
-
 Poterioochromonas malhamensis
2.4.1.30 25 30
-
 Ochromonas danica
2.4.1.30 30
-
-
 Euglena gracilis
2.4.1.31 40
-
-
 Acholeplasma laidlawii
2.4.1.31 55
-
-
 Paenibacillus sp. YM1
2.4.1.97 25
-
-
 Ochromonas danica
2.4.1.97 75
-
-
 Thermosipho africanus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.30 1.1
-
 D-glucose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.30 1.1
-
 laminaribiose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.30 1.1
-
 laminaritriose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.30 1.1
-
 laminaritetraose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.30 1.1
-
 Laminaripentaose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.30 1.1
-
 Laminarihexaose pH 6.3-6.9, 30°C Euglena gracilis
2.4.1.31 1
-
 2-deoxy-D-glucose pH 6.0, 40°C Acholeplasma laidlawii
2.4.1.31 1.4
-
 D-glucose pH 6.0, 40°C Acholeplasma laidlawii
2.4.1.31 15
-
 D-glucose pH 6.8-7.0, 55°C Paenibacillus sp. YM1
2.4.1.97 1.4
-
 xylose pH 7.5, 75°C Thermosipho africanus
2.4.1.97 7.6
-
 alpha-D-glucose 1-phosphate pH 7.5, 75°C Thermosipho africanus
2.4.1.97 27
-
 Laminaripentaose pH and temperature not specified in the publication Paenibacillus polymyxa
2.4.1.97 30
-
 Laminarihexaose pH and temperature not specified in the publication Paenibacillus polymyxa
2.4.1.97 30.3
-
 D-glucose pH 7.5, 75°C Thermosipho africanus
2.4.1.97 32
-
 laminaribiose pH and temperature not specified in the publication Paenibacillus polymyxa
2.4.1.97 33
-
 laminaritriose pH and temperature not specified in the publication Paenibacillus polymyxa
2.4.1.97 33
-
 laminaritetraose pH and temperature not specified in the publication Paenibacillus polymyxa
2.4.1.97 361
-
 cellobiose pH 7.5, 75°C Thermosipho africanus
2.4.1.97 527
-
 cellopentaose pH 7.5, 75°C Thermosipho africanus
2.4.1.97 603
-
 cellotriose pH 7.5, 75°C Thermosipho africanus
2.4.1.97 612
-
 cellotetraose pH 7.5, 75°C Thermosipho africanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.30 5.5
-
-
 Ochromonas danica
2.4.1.30 5.5
-
-
 Poterioochromonas malhamensis
2.4.1.30 6.3 6.9
-
 Euglena gracilis
2.4.1.31 6
-
-
 Acholeplasma laidlawii
2.4.1.31 6.8 7
-
 Paenibacillus sp. YM1
2.4.1.97 5.5
-
-
 Ochromonas danica
2.4.1.97 7.5
-
-
 Thermosipho africanus

General Information

EC Number General Information Comment Organism
2.4.1.30 evolution enzyme BOP belongs to the glycoside family 94, GH94 Ochromonas danica
2.4.1.30 evolution enzyme BOP belongs to the glycoside family 94, GH94 Poterioochromonas malhamensis
2.4.1.30 evolution the enzyme belongs to glycoside family 149, GH149 Euglena gracilis
2.4.1.30 metabolism beta-glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of beta-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of beta-glucans as major bioactive compounds. Three different phosphorylase specificities have been described that involve the disaccharide laminaribiose or beta-1,3-glucans, i.e., laminaribiose phosphorylase (LBP, EC 2.4.1.31), beta-1,3-oligoglucan phosphorylase (BOP, EC 2.4.1.30), and beta-1,3-polyglucan or laminarin phosphorylase (BGP, EC 2.4.1.97). Although all can degrade the characteristic beta-1,3-glycosidic linkage, they exhibit a different preference for the chain length of their substrate Ochromonas danica
2.4.1.30 metabolism beta-glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of beta-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of beta-glucans as major bioactive compounds. Three different phosphorylase specificities have been described that involve the disaccharide laminaribiose or beta-1,3-glucans, i.e., laminaribiose phosphorylase (LBP, EC 2.4.1.31), beta-1,3-oligoglucan phosphorylase (BOP, EC 2.4.1.30), and beta-1,3-polyglucan or laminarin phosphorylase (BGP, EC 2.4.1.97). Although all can degrade the characteristic beta-1,3-glycosidic linkage, they exhibit a different preference for the chain length of their substrate Poterioochromonas malhamensis
2.4.1.30 metabolism beta-glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of beta-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of beta-glucans as major bioactive compounds. Three different phosphorylase specificities have been described that involve the disaccharide laminaribiose or beta-1,3-glucans, i.e., laminaribiose phosphorylase (LBP, EC 2.4.1.31), beta-1,3-oligoglucan phosphorylase (BOP, EC 2.4.1.30), and beta-1,3-polyglucan or laminarin phosphorylase (BGP, EC 2.4.1.97). Although all can degrade the characteristic beta-1,3-glycosidic linkage, they exhibit a different preference for the chain length of their substrate Euglena gracilis
2.4.1.31 evolution enzyme LBP belongs to the glycoside family 94, GH94 Acholeplasma laidlawii
2.4.1.31 evolution enzyme LBP belongs to the glycoside family 94, GH94 Paenibacillus sp. YM1
2.4.1.31 metabolism beta-glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of beta-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of beta-glucans as major bioactive compounds. Three different phosphorylase specificities have been described that involve the disaccharide laminaribiose or beta-1,3-glucans, i.e., laminaribiose phosphorylase (LBP, EC 2.4.1.31), beta-1,3-oligoglucan phosphorylase (BOP, EC 2.4.1.30), and beta-1,3-polyglucan or laminarin phosphorylase (BGP, EC 2.4.1.97). Although all can degrade the characteristic beta-1,3-glycosidic linkage, they exhibit a different preference for the chain length of their substrate Acholeplasma laidlawii
2.4.1.31 metabolism beta-glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of beta-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of beta-glucans as major bioactive compounds. Three different phosphorylase specificities have been described that involve the disaccharide laminaribiose or beta-1,3-glucans, i.e., laminaribiose phosphorylase (LBP, EC 2.4.1.31), beta-1,3-oligoglucan phosphorylase (BOP, EC 2.4.1.30), and beta-1,3-polyglucan or laminarin phosphorylase (BGP, EC 2.4.1.97). Although all can degrade the characteristic beta-1,3-glycosidic linkage, they exhibit a different preference for the chain length of their substrate Paenibacillus sp. YM1
2.4.1.97 evolution the enzyme belongs to the glycosylhydrolase family 161, GH161 Thermosipho africanus
2.4.1.97 evolution the enzyme belongs to the glycosylhydrolase family 161, GH161 Paenibacillus polymyxa
2.4.1.97 evolution the enzyme belongs to the glycosylhydrolase family 161, GH161 Ochromonas danica
2.4.1.97 metabolism beta-glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of beta-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of beta-glucans as major bioactive compounds. Three different phosphorylase specificities have been described that involve the disaccharide laminaribiose or beta-1,3-glucans, i.e. laminaribiose phosphorylase (LBP, EC 2.4.1.31), beta-1,3-oligoglucan phosphorylase (BOP, EC 2.4.1.30), and beta-1,3-polyglucan or laminarin phosphorylase (BGP, EC 2.4.1.97). Although all can degrade the characteristic beta-1,3-glycosidic linkage, they exhibit a different preference for the chain length of their substrate Thermosipho africanus
2.4.1.97 metabolism beta-glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of beta-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of beta-glucans as major bioactive compounds. Three different phosphorylase specificities have been described that involve the disaccharide laminaribiose or beta-1,3-glucans, i.e. laminaribiose phosphorylase (LBP, EC 2.4.1.31), beta-1,3-oligoglucan phosphorylase (BOP, EC 2.4.1.30), and beta-1,3-polyglucan or laminarin phosphorylase (BGP, EC 2.4.1.97). Although all can degrade the characteristic beta-1,3-glycosidic linkage, they exhibit a different preference for the chain length of their substrate Paenibacillus polymyxa
2.4.1.97 metabolism beta-glucan phosphorylases are carbohydrate-active enzymes that catalyze the reversible degradation of beta-linked glucose polymers, with outstanding potential for the biocatalytic bottom-up synthesis of beta-glucans as major bioactive compounds. Three different phosphorylase specificities have been described that involve the disaccharide laminaribiose or beta-1,3-glucans, i.e. laminaribiose phosphorylase (LBP, EC 2.4.1.31), beta-1,3-oligoglucan phosphorylase (BOP, EC 2.4.1.30), and beta-1,3-polyglucan or laminarin phosphorylase (BGP, EC 2.4.1.97). Although all can degrade the characteristic beta-1,3-glycosidic linkage, they exhibit a different preference for the chain length of their substrate Ochromonas danica