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Literature summary extracted from

  • Dong, S.; Nhu-Lam, M.; Nagarajan, R.; Nair, S.
    Structure-guided biochemical analysis of quorum signal synthase specificities (2020), ACS Chem. Biol., 15, 1497-1504.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.184 structure to 1.93 A resolution Mesorhizobium sp. ORS 3359
2.3.1.229 in complex with CoA and p-coumaroyl S-adenosyl-L-homocysteine, to 1.85 A resolution. The CoA binding pocket is lined with hydrophobic residues, including Ile 28, Tyr29, Trp35, Val106, and Trp147. The indole platform comprises residues Trp146 and Trp147 Rhodopseudomonas palustris

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.184 F147Y variant produces about 80% C4-homoserine lactone and 20% isovaleryl-homoserinelactone when incubated with with the corresponding acyl-CoAs Mesorhizobium sp. ORS 3359
2.3.1.184 L153A variant produces about 70% C8-homoserine lactone and 30% C6-homoserinelactone when incubated with with the corresponding acyl-CoAs Mesorhizobium sp. ORS 3359
2.3.1.184 L153F variant produces about 65% C4-homoserine lactone and less than 35% C6-homoserinelactone when incubated with with the corresponding acyl-CoAs Mesorhizobium sp. ORS 3359
2.3.1.184 W105Y variant produces about 70% C4-homoserine lactone and 30% C12-homoserine lactone when incubated with with the corresponding acyl-CoAs Mesorhizobium sp. ORS 3359
2.3.1.229 Q124A mutant shows increased activity with 4-O-methylcinnamoyl-CoA, yielding about 80% N-(4-O-methylcinnamoyl)-L-homoserine lactone Rhodopseudomonas palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.184 0.0045
-
 hexanoyl-[acp] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0063
-
 decanoyl-[acp] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0073
-
 octanoyl-[acp] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0091
-
 lauroyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0103
-
 5-methylhexanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0143
-
 3-methylvaleryl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0188
-
 4-methylvaleryl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0217
-
 butanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0238
-
 3-cyclopropylbutanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0339
-
 isobutanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0344
-
 isovaleryl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.1 2 4-coumaroyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.229 0.0026
-
 4-coumaroyl-CoA pH not specified in the publication, temperature not specified in the publication Rhodopseudomonas palustris

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.184 Mesorhizobium sp. ORS 3359 A0A090F1W4
-
-
2.3.1.229 Rhodopseudomonas palustris Q6NCZ6
-
-
2.3.1.229 Rhodopseudomonas palustris ATCC BAA-98 Q6NCZ6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.184 3-cyclopropylbutanoyl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-3-cyclopropylbutanoyl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.184 3-methylvaleryl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-3-methylvaleryl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.184 4-coumaroyl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-4-coumaroyl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.184 4-methylvaleryl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-4-methylvaleryl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.184 5-methylhexanoyl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-5-mehylhexanoyl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.184 butanoyl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-butanoyl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.184 decanoyl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-butdecanoyl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.184 hexanoyl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-hexanoyl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.184 isobutanoyl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-isobutanoyl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.184 isovaleryl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-isovaleryl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.184 lauroyl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-lauroyl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.184 octanoyl-[ACP] + S-adenosyl-L-methionine
-
 Mesorhizobium sp. ORS 3359 N-octanoyl-L-homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H+
-
 ?
2.3.1.229 4-coumaroyl-CoA + S-adenosyl-L-methionine
-
 Rhodopseudomonas palustris CoA + S-methyl-5'-thioadenosine + N-(4-coumaroyl)-L-homoserine lactone
-
 ?
2.3.1.229 4-coumaroyl-CoA + S-adenosyl-L-methionine
-
 Rhodopseudomonas palustris ATCC BAA-98 CoA + S-methyl-5'-thioadenosine + N-(4-coumaroyl)-L-homoserine lactone
-
 ?
2.3.1.229 4-O-methylcinnamoyl-CoA + S-adenosyl-L-methionine
-
 Rhodopseudomonas palustris CoA + S-methyl-5'-thioadenosine + N-(4-O-methylcinnamoyl)-L-homoserine lactone
-
 ?
2.3.1.229 4-O-methylcinnamoyl-CoA + S-adenosyl-L-methionine
-
 Rhodopseudomonas palustris ATCC BAA-98 CoA + S-methyl-5'-thioadenosine + N-(4-O-methylcinnamoyl)-L-homoserine lactone
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.184 MesI
-
 Mesorhizobium sp. ORS 3359
2.3.1.229 RpaI
-
 Rhodopseudomonas palustris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.184 0.001
-
 3-cyclopropylbutanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0011
-
 decanoyl-[acp] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0017
-
 lauroyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0017
-
 4-coumaroyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0019
-
 3-methylvaleryl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0022
-
 isovaleryl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0027
-
 4-methylvaleryl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0048
-
 octanoyl-[acp] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0084
-
 5-methylhexanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0093
-
 hexanoyl-[acp] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.0099
-
 butanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.017
-
 isobutanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.184 0.02
-
 4-coumaroyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.04
-
 3-cyclopropylbutanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.06
-
 isovaleryl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.082
-
 5-methylhexanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.14
-
 3-methylvaleryl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.15
-
 4-methylvaleryl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.17
-
 decanoyl-[acp] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.19
-
 lauroyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.46
-
 butanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.5
-
 isobutanoyl-[ACP] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 0.66
-
 octanoyl-[acp] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359
2.3.1.184 2.17
-
 hexanoyl-[acp] pH not specified in the publication, temperature not specified in the publication Mesorhizobium sp. ORS 3359