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Literature summary extracted from

  • Regnard, C.; Desbruyeres, E.; Denoulet, P.; Edde, B.
    Tubulin polyglutamylase isozymic variants and regulation during the cell cycle in HeLa cells (1999), J. Cell Sci., 112, 4281-4289 .
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.61 Mg2+ 8 mM used in assay conditions Mus musculus
6.3.2.62 Mg2+ 8 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.61 n ATP + [alpha-tubulin]-L-glutamate + n L-glutamate Mus musculus overall reaction [alpha-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
 ?
6.3.2.61 n ATP + [beta-tubulin]-L-glutamate + n L-glutamate Mus musculus overall reaction [beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
 ?
6.3.2.62 n ATP + [beta-tubulin]-L-glutamate + n L-glutamate Homo sapiens overall reaction [beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.61 Mus musculus
-
-
-
6.3.2.62 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.61 partially purified by phosphocellulose column chromatography Mus musculus
6.3.2.62 partially purified by phosphocellulose column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.61 brain
-
 Mus musculus
-
6.3.2.62 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.61 additional information the enzyme is able to glutamylate both alpha- and beta-tubulin from mouse brain but only alpha-tubulin from HeLa cells Mus musculus ?
-
 ?
6.3.2.61 n ATP + [alpha-tubulin]-L-glutamate + n L-glutamate overall reaction Mus musculus [alpha-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
 ?
6.3.2.61 n ATP + [beta-tubulin]-L-glutamate + n L-glutamate overall reaction Mus musculus [beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
 ?
6.3.2.62 additional information the enzyme does not glutamylate alpha-tubulin Homo sapiens ?
-
 ?
6.3.2.62 n ATP + [beta-tubulin]-L-glutamate + n L-glutamate overall reaction Homo sapiens [beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.61 alpha-TPG
-
 Mus musculus
6.3.2.61 tubulin polyglutamylase
-
 Mus musculus
6.3.2.62 beta-TPG
-
 Homo sapiens
6.3.2.62 tubulin polyglutamylase
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
6.3.2.61 Mus musculus enzyme activity is maximal in G2-phase and decreases during M-phase of the cell cycle up
6.3.2.62 Homo sapiens enzyme activity is maximal in G2-phase and decreases during M-phase of the cell cycle up