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Literature summary extracted from

  • Chien, M.H.; Lee, W.J.; Yang, Y.C.; Tan, P.; Pan, K.F.; Liu, Y.C.; Tsai, H.C.; Hsu, C.H.; Wen, Y.C.; Hsiao, M.; Hua, K.T.
    N-alpha-acetyltransferase 10 protein promotes metastasis by stabilizing matrix metalloproteinase-2 protein in human osteosarcomas (2018), Cancer Lett., 433, 86-98 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.255 gene NAA10, recombinant expression of the enzyme in human 293T cells using a lentiviral vector, recombinant expression of GST-tagged wild-type and mutant Naa10 enzymes in Escherichia coli strain BL21 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.255 additional information knockdown of Naa10p by shRNAs, knockdown efficiencies, overview. Generation of truncated Naa10p mutants Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.255 Homo sapiens P41227
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.255 recombinant GST-tagged wild-type and mutant Naa10 enzymes from Escherichia coli strain BL21 by glutathione affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.255 143-B cell
-
 Homo sapiens
-
2.3.1.255 bone
-
 Homo sapiens
-
2.3.1.255 HOS cell
-
 Homo sapiens
-
2.3.1.255 MG-63 cell
-
 Homo sapiens
-
2.3.1.255 MNNG/HOS cell
-
 Homo sapiens
-
2.3.1.255 additional information enzyme levels in different osteosarcoma cell lines, overview Homo sapiens
-
2.3.1.255 osteoblast located around new bone surfaces Homo sapiens
-
2.3.1.255 osteosarcoma cell higher NAA10 transcripts in metastatic osteosarcoma tissues compared to non-metastatic tissues. MMP-2 expression levels are also significantly correlated with Naa10p levels in osteosarcoma tissues Homo sapiens
-
2.3.1.255 SAOS-2 cell
-
 Homo sapiens
-
2.3.1.255 U2-OS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.255 acetyl-CoA + N-terminal L-methionyl-[MMP2] matrix metalloproteinase-2, MMP2, with sequence MEALMAR Homo sapiens CoA + H+ + N-terminal Nalpha-acetyl-L-methionyl-[MMP2]
-
 ?
2.3.1.255 additional information no activity with an MMP2 mutated at the acetylytion site of Naa10 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.255 N-alpha-acetyltransferase 10
-
 Homo sapiens
2.3.1.255 NAA10
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.255 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.255 8
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.255 acetyl-CoA
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.255 malfunction knockdown and overexpression of Naa10p in osteosarcoma cells respectively leads to decreased and increased cell migratory/invasive abilities. Re-expression of Naa10p, but not of an enzymatically inactive mutant, relieves suppression of the invasive ability in vitro and metastasis in vivo imposed by Naa10p-knockdown. The matrix metalloproteinase (MMP)-2 is responsible for the Naa10p-induced invasive phenotype Homo sapiens
2.3.1.255 physiological function N-alpha-acetyltransferase 10 protein (Naa10p) mediates N-terminal acetylation of nascent proteins. It promotes metastasis by stabilizing matrix metalloproteinase-2 protein in human osteosarcomas via its N-terminal acetylation activity. Oncogenic role of Naa10p, overview. Higher NAA10 transcripts are observed in metastatic osteosarcoma tissues compared to non-metastatic tissues and are also correlated with a worse prognosis of patients. Naa10p is directly associated with MMP-2 protein through its acetyltransferase domain and maintains MMP-2 protein stability via NatA complex activity. MMP-2 expression levels are also significantly correlated with Naa10p levels in osteosarcoma tissues. Function of Naa10p in the regulation of cell invasiveness by preventing MMP-2 protein degradation that is crucial during osteosarcoma metastasis. Naa10p promotes migratory/invasive abilities of osteosarcoma cells, it regulates cell invasion of the osteosarcoma cell lines Homo sapiens