BRENDA - Enzyme Database

Biochemical characterization of pantoate kinase, a novel enzyme necessary for coenzyme A biosynthesis in the Archaea

Tomita, H.; Yokooji, Y.; Ishibashi, T.; Imanaka, T.; Atomi, H.; J. Bacteriol. 194, 5434-5443 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.7.1.169
expression in Thermococcus kodakarensis
Thermococcus kodakarensis
Engineering
EC Number
Protein Variants
Commentary
Organism
2.7.1.169
D143
residue is the base responsible for proton abstraction from the pantoate hydroxy group
Thermococcus kodakarensis
2.7.1.169
E134A
residue is involved in binding with Mg2+
Thermococcus kodakarensis
2.7.1.169
H131A
residue is involved in pantoate recognition. 77% of wild-type activity in presence of 6 mM pantoate and 4 mM ATP
Thermococcus kodakarensis
2.7.1.169
R155A
residue is involved in pantoate recognition and plays an important role in catalysis
Thermococcus kodakarensis
2.7.1.169
S104A
residue is involved in binding with phosphate
Thermococcus kodakarensis
2.7.1.169
S28A
residue is involved in pantoate recognition. 3.7% of wild-type activity in presence of 6 mM pantoate and 4 mM ATP
Thermococcus kodakarensis
2.7.1.169
T186A
residue is involved in pantoate recognition and plays an important role in catalysis. Thr186 is involved in dimer assambly
Thermococcus kodakarensis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.169
additional information
not inhibitory: CoA, acetyl-CoA
Thermococcus kodakarensis
2.7.1.169
Pantoate
substrate inhibition
Thermococcus kodakarensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.17
-
UTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
0.32
-
ATP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
0.34
-
CTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
0.43
-
GTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
2.92
-
Pantoate
pH 7.5, 42°C
Thermococcus kodakarensis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.169
K+
moderate stimulation, optimum at 10 mM
Thermococcus kodakarensis
2.7.1.169
Mg2+
required
Thermococcus kodakarensis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.1.169
Thermococcus kodakarensis
Q5JHF1
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.1.169
ATP + pantoate
reaction displays Michaelis-Menten kinetics toward ATP, whereas substrate inhibition is observed with pantoate
722549
Thermococcus kodakarensis
ADP + 4-phosphopantoate
-
-
-
?
2.7.1.169
CTP + pantoate
-
722549
Thermococcus kodakarensis
CDP + 4-phosphopantoate
-
-
-
?
2.7.1.169
GTP + pantoate
-
722549
Thermococcus kodakarensis
GDP + 4-phosphopantoate
-
-
-
?
2.7.1.169
additional information
enzyme displays broad nucleotide specificity and utilizes ATP, GTP, UTP, and CTP with comparable kcat/Km values
722549
Thermococcus kodakarensis
?
-
-
-
?
2.7.1.169
UTP + pantoate
-
722549
Thermococcus kodakarensis
UDP + 4-phosphopantoate
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.7.1.169
PoK
-
Thermococcus kodakarensis
2.7.1.169
TK2141
-
Thermococcus kodakarensis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.169
80
-
-
Thermococcus kodakarensis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.32
-
GTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
0.47
-
CTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
1.11
-
UTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
1.48
-
ATP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
2.82
-
Pantoate
pH 7.5, 42°C
Thermococcus kodakarensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.169
7.5
-
-
Thermococcus kodakarensis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.169
9.75
-
Pantoate
pH 7.5, 42°C
Thermococcus kodakarensis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.169
expression in Thermococcus kodakarensis
Thermococcus kodakarensis
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
2.7.1.169
D143
residue is the base responsible for proton abstraction from the pantoate hydroxy group
Thermococcus kodakarensis
2.7.1.169
E134A
residue is involved in binding with Mg2+
Thermococcus kodakarensis
2.7.1.169
H131A
residue is involved in pantoate recognition. 77% of wild-type activity in presence of 6 mM pantoate and 4 mM ATP
Thermococcus kodakarensis
2.7.1.169
R155A
residue is involved in pantoate recognition and plays an important role in catalysis
Thermococcus kodakarensis
2.7.1.169
S104A
residue is involved in binding with phosphate
Thermococcus kodakarensis
2.7.1.169
S28A
residue is involved in pantoate recognition. 3.7% of wild-type activity in presence of 6 mM pantoate and 4 mM ATP
Thermococcus kodakarensis
2.7.1.169
T186A
residue is involved in pantoate recognition and plays an important role in catalysis. Thr186 is involved in dimer assambly
Thermococcus kodakarensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.169
additional information
not inhibitory: CoA, acetyl-CoA
Thermococcus kodakarensis
2.7.1.169
Pantoate
substrate inhibition
Thermococcus kodakarensis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.169
9.75
-
Pantoate
pH 7.5, 42°C
Thermococcus kodakarensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.17
-
UTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
0.32
-
ATP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
0.34
-
CTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
0.43
-
GTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
2.92
-
Pantoate
pH 7.5, 42°C
Thermococcus kodakarensis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.169
K+
moderate stimulation, optimum at 10 mM
Thermococcus kodakarensis
2.7.1.169
Mg2+
required
Thermococcus kodakarensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.1.169
ATP + pantoate
reaction displays Michaelis-Menten kinetics toward ATP, whereas substrate inhibition is observed with pantoate
722549
Thermococcus kodakarensis
ADP + 4-phosphopantoate
-
-
-
?
2.7.1.169
CTP + pantoate
-
722549
Thermococcus kodakarensis
CDP + 4-phosphopantoate
-
-
-
?
2.7.1.169
GTP + pantoate
-
722549
Thermococcus kodakarensis
GDP + 4-phosphopantoate
-
-
-
?
2.7.1.169
additional information
enzyme displays broad nucleotide specificity and utilizes ATP, GTP, UTP, and CTP with comparable kcat/Km values
722549
Thermococcus kodakarensis
?
-
-
-
?
2.7.1.169
UTP + pantoate
-
722549
Thermococcus kodakarensis
UDP + 4-phosphopantoate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.169
80
-
-
Thermococcus kodakarensis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.32
-
GTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
0.47
-
CTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
1.11
-
UTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
1.48
-
ATP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
2.82
-
Pantoate
pH 7.5, 42°C
Thermococcus kodakarensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.169
7.5
-
-
Thermococcus kodakarensis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.74
-
GTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
1.4
-
CTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
2.2
-
ATP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
6.5
-
UTP
pH 7.5, 42°C
Thermococcus kodakarensis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.169
0.74
-
GTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
1.4
-
CTP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
2.2
-
ATP
pH 7.5, 42°C
Thermococcus kodakarensis
2.7.1.169
6.5
-
UTP
pH 7.5, 42°C
Thermococcus kodakarensis