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Literature summary extracted from

  • van der Wel, H.; Johnson, J.M.; Xu, Y.; Karunaratne, C.V.; Wilson, K.D.; Vohra, Y.; Boons, G.J.; Taylor, C.M.; Bendiak, B.; West, C.M.
    Requirements for Skp1 processing by cytosolic prolyl 4(trans)-hydroxylase and alpha-N-acetylglucosaminyltransferase enzymes involved in O2 signaling in dictyostelium (2011), Biochemistry, 50, 1700-1713.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.229 cloning of a hybrid Gnt1, the coding sequence is generated from the 16 amino acid exon 1 of Dictyostelium discoideum Gnt1 and exon 2 of Dictyostelium purpureum Gnt1, and expression in Escherichia coli as His-tagged protein Dictyostelium discoideum
2.4.1.229 cloning of a hybrid Gnt1, the coding sequence is generated from the 16 amino acid exon 1 of Dictyostelium discoideum Gnt1 and exon 2 of Dictyostelium purpureum Gnt1, and expression in Escherichia coli as His-tagged protein Dictyostelium purpureum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.229 UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline Dictyostelium discoideum
-
 UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?
2.4.1.229 UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline Dictyostelium purpureum
-
 UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.229 Dictyostelium discoideum
-
-
-
2.4.1.229 Dictyostelium purpureum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.229 recombinant His-tagged hybrid Gnt1, formed of Dictyostelium discoideum Gnt1/Dictyostelium purpureum Gnt1, from Escherichia coli by nickel affinity chromatography Dictyostelium purpureum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.229 additional information substrate specificity of recombinant Gnt1 hydrid enzyme, although synthetic 13-mer and 23-mer hydroxyproline peptides are substrates, their activities are much lower even at 5000fold higher concentration, the 40-mer hydroxyproline peptide is also a poor substrate, overview Dictyostelium discoideum ?
-
 ?
2.4.1.229 additional information substrate specificity of recombinant Gnt1 hydrid enzyme, although synthetic 13-mer and 23-mer hydroxyproline peptides are substrates than Skyp1A, their activities are much lower even at 5000fold higher concentration, the 40-mer hydroxyproline peptide is also a poor substrate, overview Dictyostelium purpureum ?
-
 ?
2.4.1.229 UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
-
 Dictyostelium discoideum UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?
2.4.1.229 UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
-
 Dictyostelium purpureum UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?
2.4.1.229 UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline transfer to the hydroxyproline, at the Thr-Pro143 dipeptide, preceded by a coil and succeeded by an alpha-helix in Skp1 Dictyostelium discoideum UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?
2.4.1.229 UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline transfer to the hydroxyproline, at the Thr-Pro143 dipeptide, preceded by a coil and succeeded by an alpha-helix in Skp1 Dictyostelium purpureum UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.229 GnT1
-
 Dictyostelium discoideum
2.4.1.229 GnT1
-
 Dictyostelium purpureum
2.4.1.229 hydroxyproline-capping enzyme
-
 Dictyostelium discoideum
2.4.1.229 hydroxyproline-capping enzyme
-
 Dictyostelium purpureum

General Information

EC Number General Information Comment Organism
2.4.1.229 metabolism a hypoxia inducible factor-alpha (HIFR) type prolyl 4-hydroxylase, P4H1, and an alpha-N-acetylglucosaminyltransferase Gnt1, sequentially modify proline 143 of Skp1, a subunit of the Skp1/Cullin/F-box protein, SCF, class of E3 ubiquitin ligases Dictyostelium discoideum
2.4.1.229 metabolism a hypoxia inducible factor-alpha (HIFR) type prolyl 4-hydroxylase, P4H1, and an alpha-N-acetylglucosaminyltransferase Gnt1, sequentially modify proline 143 of Skp1, a subunit of the Skp1/Cullin/F-box protein, SCF, class of E3 ubiquitin ligases Dictyostelium purpureum
2.4.1.229 additional information Gnt1 from Dictyostelium discoideum contains a stretch of 80 Asn residues and other Asn-rich inserts Dictyostelium discoideum
2.4.1.229 additional information Gnt1 from Dictyostelium purureum lacks a stretch of 80 Asn residues and other Asn-rich inserts Dictyostelium purpureum