Literature summary extracted from

Fonknechten, N.; Perret, A.; Perchat, N.; Tricot, S.; Lechaplais, C.; Vallenet, D.; Vergne, C.; Zaparucha, A.; Le Paslier, D.; Weissenbach, J.; Salanoubat, M.
A conserved gene cluster rules anaerobic oxidative degradation of L-ornithine (2009), J. Bacteriol., 191, 3162-3167.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.3.1.263	expression as a His-tagged protein	Acetoanaerobium sticklandii
5.1.1.12	expressed His-tagged recombinant protein	Acetoanaerobium sticklandii

Inhibitors

EC Number	Inhibitors	Comment	Organism
1.4.1.12	2-amino-4-ketopentanoate	70% inhibition in the presence of 1 mM 2-amino-4-ketopentanoate	Acetoanaerobium sticklandii
1.4.1.12	acetyl-CoA	30% inhibition in the presence of 1 mM acetyl-CoA	Acetoanaerobium sticklandii
1.4.1.12	D-alanine	45% inhibition in the presence of 1 mM D-alanine	Acetoanaerobium sticklandii

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
1.4.1.12	2	-	NADP+	-	Acetoanaerobium sticklandii
1.4.1.12	2100	-	NAD+	-	Acetoanaerobium sticklandii
2.3.1.263	0.018	-	CoA	pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate	Acetoanaerobium sticklandii
2.3.1.263	0.035	-	2-Amino-4-oxopentanoate	pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate	Acetoanaerobium sticklandii
5.1.1.12	0.52	-	L-ornithine	-	Acetoanaerobium sticklandii

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.4.1.12	38000	-	SDS-PAGE	Acetoanaerobium sticklandii
1.4.1.12	38930	-	calculated from cDNA	Acetoanaerobium sticklandii
2.3.1.263	13930	-	2 * 13930 + 2 * 51450, calculated from sequence	Acetoanaerobium sticklandii
2.3.1.263	14000	-	2 * 50000 + 2 * 14000, SDS-PAGE	Acetoanaerobium sticklandii
2.3.1.263	50000	-	2 * 50000 + 2 * 14000, SDS-PAGE	Acetoanaerobium sticklandii
2.3.1.263	51450	-	2 * 13930 + 2 * 51450, calculated from sequence	Acetoanaerobium sticklandii
2.3.1.263	136000	-	gel filtration	Acetoanaerobium sticklandii
5.1.1.12	21500	-	2 * 21500, gel filtration	Acetoanaerobium sticklandii
5.1.1.12	43000	-	SDS-PAGE	Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
1.5.1.2	DELTA1-pyrroline-5-carboxylate + NADH + H+	Acetoanaerobium sticklandii	-	L-proline + NAD+	-	r
1.21.4.1	D-proline + NADH + H+	Acetoanaerobium sticklandii	-	5-aminovalerate + NAD+	-	r
2.3.1.263	CoA + 2-amino-4-oxopentanoate	Acetoanaerobium sticklandii	catalyzes a step in the ornithine fermentation pathway	acetyl-CoA + D-alanine	-	r
2.6.1.13	L-ornithine + 2-oxoglutarate	Acetoanaerobium sticklandii	-	DELTA1-pyrroline-5-carboxylate + L-glutamate	-	r
4.3.1.12	L-ornithine	Acetoanaerobium sticklandii	-	L-proline + NH3	-	r
5.1.1.4	L-proline	Acetoanaerobium sticklandii	-	D-proline	-	r
5.1.1.12	L-Ornithine	Acetoanaerobium sticklandii	-	D-Ornithine	-	r
5.4.3.5	D-ornithine	Acetoanaerobium sticklandii	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Clostridioides difficile	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Cutibacterium acnes	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Alkaliphilus metalliredigens	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Alkaliphilus oremlandii	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Fervidobacterium nodosum Rt17-B1	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Petrotoga mobilis	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Thermoanaerobacter pseudethanolicus	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Natranaerobius thermophilus	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Thermosipho melanesiensis	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Natranaerobius thermophilus JW/NM-WN-LF	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Clostridioides difficile 630	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Thermosipho melanesiensis BI429	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Petrotoga mobilis SJ95	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Thermoanaerobacter pseudethanolicus MB4	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Cutibacterium acnes KPA171202	-	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	Alkaliphilus oremlandii OhlLAs	-	(2R,4S)-2,4-diaminopentanoate	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.4.1.12	Acetoanaerobium sticklandii	-	-	-
1.5.1.2	Acetoanaerobium sticklandii	O87725	-	-
1.21.4.1	Acetoanaerobium sticklandii	-	-	-
2.3.1.263	Acetoanaerobium sticklandii	C1FW07 and C1FW06	C1FW07: beta subunit, C1FW06: alpha subunit. No significant homology with any known enzyme family	-
2.6.1.13	Acetoanaerobium sticklandii	-	-	-
4.3.1.12	Acetoanaerobium sticklandii	-	-	-
5.1.1.4	Acetoanaerobium sticklandii	Q9L4Q3	-	-
5.1.1.12	Acetoanaerobium sticklandii	C1FW08	-	-
5.4.3.5	Acetoanaerobium sticklandii	-	strain DSM 619	-
5.4.3.5	Alkaliphilus metalliredigens	-	-	-
5.4.3.5	Alkaliphilus oremlandii	-	-	-
5.4.3.5	Alkaliphilus oremlandii OhlLAs	-	-	-
5.4.3.5	Clostridioides difficile	-	-	-
5.4.3.5	Clostridioides difficile 630	-	-	-
5.4.3.5	Cutibacterium acnes	-	-	-
5.4.3.5	Cutibacterium acnes KPA171202	-	-	-
5.4.3.5	Fervidobacterium nodosum Rt17-B1	-	-	-
5.4.3.5	Natranaerobius thermophilus	-	-	-
5.4.3.5	Natranaerobius thermophilus JW/NM-WN-LF	-	-	-
5.4.3.5	Petrotoga mobilis	-	-	-
5.4.3.5	Petrotoga mobilis SJ95	-	-	-
5.4.3.5	Thermoanaerobacter pseudethanolicus	-	-	-
5.4.3.5	Thermoanaerobacter pseudethanolicus MB4	-	-	-
5.4.3.5	Thermosipho melanesiensis	-	-	-
5.4.3.5	Thermosipho melanesiensis BI429	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.3.1.263	-	Acetoanaerobium sticklandii
5.1.1.12	-	Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
1.4.1.12	2,4-diaminopentanoate + H2O + NAD+	enzyme is more efficient with the natural substrate (2R,4S)-2,4-diaminopentanoate (Vmax: 51.6/sec) it also reacts with (2R,4R)-2,4-diaminopentanoate (Vmax: 2.8/sec)	Acetoanaerobium sticklandii	2-amino-4-oxopentanoate + NH3 + NADH	-	?
1.4.1.12	2,4-diaminopentanoate + H2O + NADP+	enzyme is more efficient with the natural substrate (2R,4S)-2,4-diaminopentanoate (Vmax: 51.6/sec) it also reacts with (2R,4R)-2,4-diaminopentanoate (Vmax: 2.8/sec)	Acetoanaerobium sticklandii	2-amino-4-oxopentanoate + NH3 + NADPH	-	?
1.5.1.2	DELTA1-pyrroline-5-carboxylate + NADH + H+	-	Acetoanaerobium sticklandii	L-proline + NAD+	-	r
1.21.4.1	D-proline + NADH + H+	-	Acetoanaerobium sticklandii	5-aminovalerate + NAD+	-	r
2.3.1.263	CoA + 2-amino-4-oxopentanoate	catalyzes a step in the ornithine fermentation pathway	Acetoanaerobium sticklandii	acetyl-CoA + D-alanine	-	r
2.3.1.263	CoA + 2-amino-4-oxopentanoate	strict substrate specificity. No activity with other proteogenic amino acid nor with propional-CoA, crotonyl-CoA, succinyl-CoA, butyryl-CoA or malonyl-CoA. In the reverse reaction formation of CoA-SH is observed only in the presence of D-alanine as substrate and not in that of the L-isomer	Acetoanaerobium sticklandii	acetyl-CoA + D-alanine	-	r
2.6.1.13	L-ornithine + 2-oxoglutarate	-	Acetoanaerobium sticklandii	DELTA1-pyrroline-5-carboxylate + L-glutamate	-	r
4.3.1.12	L-ornithine	-	Acetoanaerobium sticklandii	L-proline + NH3	-	r
5.1.1.4	L-proline	-	Acetoanaerobium sticklandii	D-proline	-	r
5.1.1.12	D-alanine	conversion is lower than 10% compared to conversion of L-ornithine	Acetoanaerobium sticklandii	L-alanine	-	r
5.1.1.12	L-asparagine	conversion is lower than 10% compared to conversion of L-ornithine	Acetoanaerobium sticklandii	D-asparagine	-	r
5.1.1.12	L-Ornithine	-	Acetoanaerobium sticklandii	D-Ornithine	-	r
5.1.1.12	L-serine	conversion is 10% compared to conversion of L-ornithine	Acetoanaerobium sticklandii	D-serine	-	r
5.4.3.5	D-ornithine	-	Acetoanaerobium sticklandii	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Clostridioides difficile	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Cutibacterium acnes	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Alkaliphilus metalliredigens	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Alkaliphilus oremlandii	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Fervidobacterium nodosum Rt17-B1	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Petrotoga mobilis	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Thermoanaerobacter pseudethanolicus	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Natranaerobius thermophilus	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Thermosipho melanesiensis	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Natranaerobius thermophilus JW/NM-WN-LF	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Clostridioides difficile 630	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Thermosipho melanesiensis BI429	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Petrotoga mobilis SJ95	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Thermoanaerobacter pseudethanolicus MB4	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Cutibacterium acnes KPA171202	(2R,4S)-2,4-diaminopentanoate	-	r
5.4.3.5	D-ornithine	-	Alkaliphilus oremlandii OhlLAs	(2R,4S)-2,4-diaminopentanoate	-	r

Subunits

EC Number	Subunits	Comment	Organism
1.4.1.12	homodimer	gel filtration, 2 * 38000 Da	Acetoanaerobium sticklandii
2.3.1.263	heterotetramer	2 * 50000 + 2 * 14000, SDS-PAGE	Acetoanaerobium sticklandii
2.3.1.263	tetramer	2 * 13930 + 2 * 51450, calculated from sequence	Acetoanaerobium sticklandii
5.1.1.12	homodimer	2 * 21500, gel filtration	Acetoanaerobium sticklandii

Synonyms

EC Number	Synonyms	Comment	Organism
1.4.1.12	2R,4S-2,4-diaminopentanoate dehydrogenase	-	Acetoanaerobium sticklandii
1.4.1.12	DAPDH	-	Acetoanaerobium sticklandii
1.5.1.2	pyrroline-5-carboxylate reductase	-	Acetoanaerobium sticklandii
1.21.4.1	D-proline reductase	-	Acetoanaerobium sticklandii
2.3.1.263	AKP thiolase	-	Acetoanaerobium sticklandii
2.3.1.263	AKPT	-	Acetoanaerobium sticklandii
2.6.1.13	ornithine transaminase	-	Acetoanaerobium sticklandii
4.3.1.12	ornithine cyclodeaminase	-	Acetoanaerobium sticklandii
5.1.1.4	proline racemase	-	Acetoanaerobium sticklandii
5.1.1.12	L-ornithine racemase	-	Acetoanaerobium sticklandii
5.1.1.12	or-5	-	Acetoanaerobium sticklandii
5.4.3.5	D-ornithine aminomutase	-	Acetoanaerobium sticklandii
5.4.3.5	D-ornithine aminomutase	-	Clostridioides difficile
5.4.3.5	D-ornithine aminomutase	-	Cutibacterium acnes
5.4.3.5	D-ornithine aminomutase	-	Alkaliphilus metalliredigens
5.4.3.5	D-ornithine aminomutase	-	Alkaliphilus oremlandii
5.4.3.5	D-ornithine aminomutase	-	Fervidobacterium nodosum Rt17-B1
5.4.3.5	D-ornithine aminomutase	-	Petrotoga mobilis
5.4.3.5	D-ornithine aminomutase	-	Thermoanaerobacter pseudethanolicus
5.4.3.5	D-ornithine aminomutase	-	Natranaerobius thermophilus
5.4.3.5	D-ornithine aminomutase	-	Thermosipho melanesiensis
5.4.3.5	oraE	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE and or-4, or-5 is placed elsewhere in the genome	Natranaerobius thermophilus
5.4.3.5	oraE	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4 and or-5 are placed elsewhere in the genome	Fervidobacterium nodosum Rt17-B1
5.4.3.5	oraE	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4 and or-5 are placed elsewhere in the genome	Petrotoga mobilis
5.4.3.5	oraE	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4 and or-5 are placed elsewhere in the genome	Thermosipho melanesiensis
5.4.3.5	oraE	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Acetoanaerobium sticklandii
5.4.3.5	oraE	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Clostridioides difficile
5.4.3.5	oraE	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Cutibacterium acnes
5.4.3.5	oraE	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Alkaliphilus metalliredigens
5.4.3.5	oraE	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Alkaliphilus oremlandii
5.4.3.5	oraE	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Thermoanaerobacter pseudethanolicus
5.4.3.5	oraS	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Acetoanaerobium sticklandii
5.4.3.5	oraS	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Clostridioides difficile
5.4.3.5	oraS	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Cutibacterium acnes
5.4.3.5	oraS	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Alkaliphilus metalliredigens
5.4.3.5	oraS	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Alkaliphilus oremlandii
5.4.3.5	oraS	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Fervidobacterium nodosum Rt17-B1
5.4.3.5	oraS	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Petrotoga mobilis
5.4.3.5	oraS	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Thermoanaerobacter pseudethanolicus
5.4.3.5	oraS	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Natranaerobius thermophilus
5.4.3.5	oraS	gene name, one of the two subunits, gene is in synteny with further genes of the ornithine fermentation pathway: or-1, or-2, or-4, oraS, oraE, or-4, or-5	Thermosipho melanesiensis

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.4.1.12	additional information	-	assay carried out at room temperature	Acetoanaerobium sticklandii

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
1.4.1.12	5	-	NADP+	-	Acetoanaerobium sticklandii
1.4.1.12	34	-	NAD+	-	Acetoanaerobium sticklandii
2.3.1.263	3.4	-	2-Amino-4-oxopentanoate	pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate	Acetoanaerobium sticklandii
2.3.1.263	3.5	-	CoA	pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate	Acetoanaerobium sticklandii
5.1.1.12	1660	-	L-ornithine	-	Acetoanaerobium sticklandii

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.4.1.12	9	-	assay at	Acetoanaerobium sticklandii

Cofactor

EC Number	Cofactor	Comment	Organism
1.4.1.12	NAD+	the enzyme can function with both nucleotides as hydrogen acceptors, NADP+ and NAD+	Acetoanaerobium sticklandii
1.4.1.12	NADP+	the enzyme can function with both nucleotides as hydrogen acceptors, NADP+ and NAD+	Acetoanaerobium sticklandii
1.4.1.12	pyridoxal 5'-phosphate	-	Acetoanaerobium sticklandii
2.3.1.263	pyridoxal 5'-phosphate	strictly pyridoxal 5'-phosphate-dependent enzyme	Acetoanaerobium sticklandii
5.1.1.12	pyridoxal 5'-phosphate	-	Acetoanaerobium sticklandii

General Information

EC Number	General Information	Comment	Organism
1.5.1.2	metabolism	catalyzes step 7 in the ornithine fermentation pathway	Acetoanaerobium sticklandii
1.21.4.1	metabolism	catalyzes step 9 in the ornithine fermentation pathway	Acetoanaerobium sticklandii
2.3.1.263	metabolism	catalyzes a step in the ornithine fermentation pathway	Acetoanaerobium sticklandii
2.6.1.13	metabolism	catalyzes step 5 in the ornithine fermentation pathway	Acetoanaerobium sticklandii
4.3.1.12	metabolism	catalyzes step 10 in the ornithine fermentation pathway	Acetoanaerobium sticklandii
5.1.1.4	metabolism	catalyzes step 8 in the ornithine fermentation pathway	Acetoanaerobium sticklandii
5.1.1.12	metabolism	catalyzes the first step in the ornithine fermentation pathway	Acetoanaerobium sticklandii
5.4.3.5	metabolism	catalyzes step 2 in the ornithine fermentation pathway	Acetoanaerobium sticklandii
5.4.3.5	metabolism	catalyzes step 2 in the ornithine fermentation pathway	Clostridioides difficile
5.4.3.5	metabolism	catalyzes step 2 in the ornithine fermentation pathway	Cutibacterium acnes
5.4.3.5	metabolism	catalyzes step 2 in the ornithine fermentation pathway	Alkaliphilus metalliredigens
5.4.3.5	metabolism	catalyzes step 2 in the ornithine fermentation pathway	Alkaliphilus oremlandii
5.4.3.5	metabolism	catalyzes step 2 in the ornithine fermentation pathway	Fervidobacterium nodosum Rt17-B1
5.4.3.5	metabolism	catalyzes step 2 in the ornithine fermentation pathway	Petrotoga mobilis
5.4.3.5	metabolism	catalyzes step 2 in the ornithine fermentation pathway	Thermoanaerobacter pseudethanolicus
5.4.3.5	metabolism	catalyzes step 2 in the ornithine fermentation pathway	Natranaerobius thermophilus
5.4.3.5	metabolism	catalyzes step 2 in the ornithine fermentation pathway	Thermosipho melanesiensis

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
1.4.1.12	0.016	-	NAD+	-	Acetoanaerobium sticklandii
1.4.1.12	2.56	-	NADP+	-	Acetoanaerobium sticklandii
2.3.1.263	97	-	2-Amino-4-oxopentanoate	pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate	Acetoanaerobium sticklandii
2.3.1.263	190	-	CoA	pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate	Acetoanaerobium sticklandii
5.1.1.12	3192	-	L-ornithine	-	Acetoanaerobium sticklandii