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Literature summary extracted from

  • Marechal, L.R.
    beta-1,3-Oligoglucan: orthophosphate glucosyltransferases from Euglena gracilis. II. Comparative studies between laminaribiose- and beta-1,3-oligoglucan phosphorylase (1967), Biochim. Biophys. Acta, 146, 431-442.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.30 2-mercaptoethanol activation Euglena gracilis
2.4.1.30 additional information absolute requirement for sulfhydryl groups Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.30 hydroquinone strongly inhibits in absence of 2-mercaptoethanol Euglena gracilis
2.4.1.30 iodoacetate
-
 Euglena gracilis
2.4.1.30 p-mercuribenzoate strong inhibition Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.30 (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate Euglena gracilis
-
 (1,3-beta-D-glucosyl)n + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.30 Euglena gracilis
-
-
-
2.4.1.31 Euglena gracilis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.30 (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 Euglena gracilis (1,3-beta-D-glucosyl)n + phosphate
-
 r
2.4.1.30 alpha-phenylglucoside + alpha-D-glucose 1-phosphate very low activity Euglena gracilis 1,3-beta-D-glucosyl-alpha-phenylglucoside + phosphate
-
 r
2.4.1.30 D-glucose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis laminaribiose + phosphate
-
 r
2.4.1.30 laminaribiose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis laminaritriose + phosphate
-
 r
2.4.1.30 laminaribiose + phosphate
-
 Euglena gracilis D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 laminaribiosyl-p-hydroquinone + alpha-D-glucose 1-phosphate
-
 Euglena gracilis laminaritriosyl-p-hydroquinone + phosphate
-
 r
2.4.1.30 laminaribiosyl-saligenin + alpha-D-glucose 1-phosphate best acceptor Euglena gracilis laminaritriosyl-saligenin + phosphate
-
 r
2.4.1.30 laminaripentaose + phosphate
-
 Euglena gracilis laminaritetraose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 laminaritetraose + alpha-D-glucose 1-phosphate low activity Euglena gracilis laminaripentaose + phosphate
-
 r
2.4.1.30 laminaritetraose + phosphate
-
 Euglena gracilis laminaritriose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 laminaritetraosyl-saligenin + alpha-D-glucose 1-phosphate low activity Euglena gracilis laminaripentaosyl-saligenin + phosphate
-
 r
2.4.1.30 laminaritriosyl-p-hydroquinone + alpha-D-glucose 1-phosphate low activity Euglena gracilis laminaritetraosyl-p-hydroquinone + phosphate
-
 r
2.4.1.30 laminaritriosyl-saligenin + alpha-D-glucose 1-phosphate low activity Euglena gracilis laminaritetraosyl-saligenin + phosphate
-
 ?
2.4.1.30 additional information EC 2.4.1.30 and EC 2.4.1.31 catalyze the same reaction but with different quantitative specificities, EC 2.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose, while the opposite behavior is observed with EC 2.4.1.31 i.e. laminaribiose phosphorylase Euglena gracilis ?
-
 ?
2.4.1.30 additional information alpha-methylglucoside and laminarin are no acceptors Euglena gracilis ?
-
 ?
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate
-
 Euglena gracilis D-glucose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose
-
 Euglena gracilis laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
 ?
2.4.1.31 laminaripentaose + phosphate
-
 Euglena gracilis laminaritetraose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.31 laminaritetraose + phosphate
-
 Euglena gracilis laminaritriose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.31 laminaritriose + phosphate
-
 Euglena gracilis laminaribiose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.31 additional information EC 1.4.1.31 and EC 1.4.1.30 catalyse the same reaction but with different quantitative specificity, EC 1.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose while the opposite behaviour is observed with EC 1.4.1.31 Euglena gracilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.30 additional information EC 2.4.1.30 and EC 2.4.1.31 catalyze the same reaction but with different quantitative specificity, EC 2.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose, while the opposite behavior is observed with EC 2.4.1.31 i.e. laminaribiose phosphorylase Euglena gracilis