Any feedback?
Literature summary extracted from
- Ornithine metabolism by Clostridium sticklandii. Oxidation of ornithine to 2-amino-4-ketopentanoic acid via 2,4-diaminopentanoic acid; participation of B12 coenzyme, pyridoxal phosphate, and pyridine nucleotide (1970), J. Biol. Chem., 245, 5914-5926.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.12 | 0.6 | 3.3 | NAD+ | - |
Acetoanaerobium sticklandii |  |
| 1.4.1.12 | 1.2 | 1.8 | 2,4-Diaminopentanoate | - |
Acetoanaerobium sticklandii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.12 | additional information | no stimulation of activity by Mg2+, Zn2+, Mn2+, Fe2+, Cd2+, Ni2+, Cu2+ | Acetoanaerobium sticklandii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.12 | 2,4-diaminopentanoate + H2O + NAD(P)+ | Acetoanaerobium sticklandii | - |
2-amino-4-oxopentanoate + NH3 + NAD(P)H | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.12 | Acetoanaerobium sticklandii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.1.12 | - |
Acetoanaerobium sticklandii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.12 | 68.5 | - |
- |
Acetoanaerobium sticklandii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.4.1.12 | 4°C, phosphate buffer, pH 7,5, 10-20% glycerol, one month | Acetoanaerobium sticklandii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.12 | 2,4-diaminopentanoate + H2O + NAD(P)+ | - |
Acetoanaerobium sticklandii | 2-amino-4-oxopentanoate + NH3 + NAD(P)H | - |
r | |
| 1.4.1.12 | 2,4-diaminopentanoate + H2O + NAD+ | - |
Acetoanaerobium sticklandii | 2-amino-4-oxopentanoate + NH3 + NADH | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.12 | 8.8 | - |
2,4-diaminopentanoate + NAD+ + H2O | Acetoanaerobium sticklandii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.12 | 8 | 10 | half maximal activity at pH 8.0 and 10.0, 2,4-diaminopentanoate + NAD+ + H2O | Acetoanaerobium sticklandii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.12 | NAD+ | - |
Acetoanaerobium sticklandii | |
| 1.4.1.12 | NADP+ | - |
Acetoanaerobium sticklandii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
