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BRENDA support

Literature summary for 7.6.2.9 extracted from

  • Wolters, J.; Ronnie, P.; Gul, N.; Karasawa, A.; Andy-Mark, W.; Slotboom, D.; Poolman, B.
    Ligand binding and crystal structures of the substrate-binding domain of the ABC transporter OpuA (2010), PLoS ONE, 5, e10361.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information the binding of glycine betaine to purified OpuA and OpuAC does not show any salt dependence or cooperative effects, in contrast to the transport activity Lactococcus lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization in an open and closed-liganded conformation, to 1.9 and 2.3 A resolution, respectively. Solutes like proline and carnitine bind with affinities that are 3 to 4 orders of magnitude lower than affinities of substrates glycine betaine or proline betaine. The low affinity substrates are not noticeably transported by membrane-reconstituted OpuA. The binding pocket is formed by three tryptophans coordinating the quaternary ammonium group of glycine betaine in the closed-liganded structure Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
carnitine
-
Lactococcus lactis
proline
-
Lactococcus lactis
proline betaine competitive Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis Q7DAU8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + glycine betaine/out
-
Lactococcus lactis ADP + phosphate + glycine betaine/in OpuAC is highly specific for glycine betaine and the related proline betaine ?
ATP + H2O + proline betaine/out
-
Lactococcus lactis ADP + phosphate + proline betaine/in OpuAC is highly specific for glycine betaine and the related proline betaine ?
additional information other solute like proline and carnitine bind with affinities that are 3 to 4 orders of magnitude lower than for glycine betaine or proline betaine Lactococcus lactis ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.048
-
proline betaine pH 7.0, 25°C Lactococcus lactis
0.065
-
carnitine pH 7.0, 25°C Lactococcus lactis
0.198
-
proline pH 7.0, 25°C Lactococcus lactis