Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the binding of glycine betaine to purified OpuA and OpuAC does not show any salt dependence or cooperative effects, in contrast to the transport activity | Lactococcus lactis |
Crystallization (Comment) | Organism |
---|---|
crystallization in an open and closed-liganded conformation, to 1.9 and 2.3 A resolution, respectively. Solutes like proline and carnitine bind with affinities that are 3 to 4 orders of magnitude lower than affinities of substrates glycine betaine or proline betaine. The low affinity substrates are not noticeably transported by membrane-reconstituted OpuA. The binding pocket is formed by three tryptophans coordinating the quaternary ammonium group of glycine betaine in the closed-liganded structure | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
carnitine | - |
Lactococcus lactis | |
proline | - |
Lactococcus lactis | |
proline betaine | competitive | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | Q7DAU8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + glycine betaine/out | - |
Lactococcus lactis | ADP + phosphate + glycine betaine/in | OpuAC is highly specific for glycine betaine and the related proline betaine | ? | |
ATP + H2O + proline betaine/out | - |
Lactococcus lactis | ADP + phosphate + proline betaine/in | OpuAC is highly specific for glycine betaine and the related proline betaine | ? | |
additional information | other solute like proline and carnitine bind with affinities that are 3 to 4 orders of magnitude lower than for glycine betaine or proline betaine | Lactococcus lactis | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
proline betaine | pH 7.0, 25°C | Lactococcus lactis | |
0.065 | - |
carnitine | pH 7.0, 25°C | Lactococcus lactis | |
0.198 | - |
proline | pH 7.0, 25°C | Lactococcus lactis |