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Literature summary for 7.6.2.9 extracted from

  • Chen, C.; Beattie, G.A.
    Characterization of the osmoprotectant transporter OpuC from Pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function (2007), J. Bacteriol., 189, 6901-6912.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.6.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, DH5-alpha used as host for cloning, MG1655 used as host for mutagenesis Pseudomonas syringae

Protein Variants

Protein Variants Comment Organism
additional information deletion mutants of OpuC generated, complementation of deletion mutants shown Pseudomonas syringae

Inhibitors

Inhibitors Comment Organism Structure
additional information competition assays performed, compounds inhibiting the uptake of choline and glycine betaine summarized Pseudomonas syringae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
 glycine betaine kinetics of OpuC-mediated uptake, in 50 mM of phosphate buffer containing 0.5 M of NaCl, 0.2% glucose, pH 7, low-affinity transport of choline determined Pseudomonas syringae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Pseudomonas syringae
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae Q87WH5 ATP-binding protein, gene PSPTO4575, OpuCA; pv. tomato, strain DC3000
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, recombinant protein Pseudomonas syringae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 osmoprotection assays and transport assays applied, kinetic studies with radiolabeled substrates performed, OpuC identified as sole transporter for glycine betaine and as one of multiple transporters for choline under high osmolarity, close relation to OpuC transporters of Bacillus subtilis and Listeria monocytogenes determined, cystathionine-beta-synthase domain identification and homology search performed Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Pseudomonas syringae ADP + phosphate
-
 ?
ATP + H2O + glycine betaine/out enhanced osmoprotection found to be correlated with higher capacity for choline uptake than for glycine uptake, broad substrate specificity including acetylcholine, carnitine, and proline betaine determined, cystathionine-beta-synthase domains shown to be essential for osmoregulatory function Pseudomonas syringae ADP + phosphate + glycine betaine/in
-
 ?

Synonyms

Synonyms Comment Organism
OpuC
-
 Pseudomonas syringae