Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, DH5-alpha used as host for cloning, MG1655 used as host for mutagenesis | Pseudomonas syringae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutants of OpuC generated, complementation of deletion mutants shown | Pseudomonas syringae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | competition assays performed, compounds inhibiting the uptake of choline and glycine betaine summarized | Pseudomonas syringae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
glycine betaine | kinetics of OpuC-mediated uptake, in 50 mM of phosphate buffer containing 0.5 M of NaCl, 0.2% glucose, pH 7, low-affinity transport of choline determined | Pseudomonas syringae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Pseudomonas syringae | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas syringae | Q87WH5 | ATP-binding protein, gene PSPTO4575, OpuCA; pv. tomato, strain DC3000 | - |
Purification (Comment) | Organism |
---|---|
gel filtration, recombinant protein | Pseudomonas syringae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
osmoprotection assays and transport assays applied, kinetic studies with radiolabeled substrates performed, OpuC identified as sole transporter for glycine betaine and as one of multiple transporters for choline under high osmolarity, close relation to OpuC transporters of Bacillus subtilis and Listeria monocytogenes determined, cystathionine-beta-synthase domain identification and homology search performed | Pseudomonas syringae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Pseudomonas syringae | ADP + phosphate | - |
? | |
ATP + H2O + glycine betaine/out | enhanced osmoprotection found to be correlated with higher capacity for choline uptake than for glycine uptake, broad substrate specificity including acetylcholine, carnitine, and proline betaine determined, cystathionine-beta-synthase domains shown to be essential for osmoregulatory function | Pseudomonas syringae | ADP + phosphate + glycine betaine/in | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OpuC | - |
Pseudomonas syringae |