Cloned (Comment) | Organism |
---|---|
substrate-binding domain into vector pBKB76, expression in Escherichia coli BL21 (DE3) | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
by hanging-drop method, structures of substrate-binding domain in complex with glycine betaine solved at 2.0 A resolution and in complex with glycine proline at 2.8 A resolution, structures show a substrate-binding protein-dependent-typical class II fold, structural differences of complexes occur within the ligand-binding pocket as well as across the domain-domain interface, explaining the differences in affinity of the substrate-binding domain-glycine betaine complex with KD = 0.017 mM, and substrate-binding domain-proline betaine complex with KD = 0.295 mM | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bacillus subtilis | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
substrate-binding domain, 1 * 28000, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
substrate-binding domain, to apparent homogeneity | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + glycine betaine/out | - |
Bacillus subtilis | ADP + phosphate + glycine betaine/in | - |
? | |
ATP + H2O + proline betaine/out | - |
Bacillus subtilis | ADP + phosphate + proline betaine/in | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | substrate-binding domain, 1 * 28000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
OpuA | - |
Bacillus subtilis |