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Literature summary for 7.6.2.9 extracted from

  • Horn, C.; Sohn-Boesser, L.; Breed, J.; Welte, W.; Schmitt, L.; Bremer, E.
    Molecular determinants for substrate specificity of the ligand-binding protein OpuAC from Bacillus subtilis for the compatible solutes glycine betaine and proline betaine (2006), J. Mol. Biol., 357, 592-606.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
substrate-binding domain into vector pBKB76, expression in Escherichia coli BL21 (DE3) Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
by hanging-drop method, structures of substrate-binding domain in complex with glycine betaine solved at 2.0 A resolution and in complex with glycine proline at 2.8 A resolution, structures show a substrate-binding protein-dependent-typical class II fold, structural differences of complexes occur within the ligand-binding pocket as well as across the domain-domain interface, explaining the differences in affinity of the substrate-binding domain-glycine betaine complex with KD = 0.017 mM, and substrate-binding domain-proline betaine complex with KD = 0.295 mM Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Bacillus subtilis 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
substrate-binding domain, 1 * 28000, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
substrate-binding domain, to apparent homogeneity Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + glycine betaine/out
-
Bacillus subtilis ADP + phosphate + glycine betaine/in
-
?
ATP + H2O + proline betaine/out
-
Bacillus subtilis ADP + phosphate + proline betaine/in
-
?

Subunits

Subunits Comment Organism
monomer substrate-binding domain, 1 * 28000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
OpuA
-
Bacillus subtilis