Protein Variants | Comment | Organism |
---|---|---|
additional information | substrate binding domain-less mutant | Lactococcus lactis |
W484C | mutant with 10fold increased KM for glycine betaine than the wild type enzyme | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
glycine betaine | 30ºC, pH 7.0, wild type/substrate binding domain-less mutant heterodimer | Lactococcus lactis | |
0.0019 | - |
glycine betaine | 30ºC, pH 7.0, wild type/W484C heterodimer | Lactococcus lactis | |
0.0025 | - |
glycine betaine | 30ºC, pH 7.0, wild type enzyme | Lactococcus lactis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Lactococcus lactis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 9 mM MgATP2- in the outside of the membranes activates OpuA | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the OpuA component of the wild-type enzyme runs at a lower molecular weight than that of W484C and the substrate binding domain-less mutant | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Lactococcus lactis NZ9000 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography and size-exclusion chromatography of solubilized membrane fractions | Lactococcus lactis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] = ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] | a single substrate binding domain per dimeric translocator complex is sufficient and minimally needed for transport. Within the complex with two substrate binding domains, the two domains interact in a cooperative manner and enhance the transport capacity | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + glycine betaine/out | - |
Lactococcus lactis | ADP + phosphate + glycine betaine/in | - |
? | |
ATP + H2O + glycine betaine/out | - |
Lactococcus lactis NZ9000 | ADP + phosphate + glycine betaine/in | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | dimeric OpuA is 220000 Da | Lactococcus lactis |