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Literature summary for 7.6.2.9 extracted from

  • van der Heide, T.; Stuart, M.C.A.; Poolman, B.
    On the osmotic signal and osmosensing mechanism of an ABC transport system for glycine betaine (2001), EMBO J., 20, 7022-7032.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.6.2.9

Activating Compound

Activating Compound Comment Organism Structure
anionic lipids absence of phosphatidylglycerol and/or phosphatidylserine results in trapping of the protein in an inactive state Lactococcus lactis
glycerol at 270 mM, the amount of glycine betaine taken up after 30 seconds is 30% higher than that of the iso-osmotic control sample Lactococcus lactis
additional information sucrose and D-fructose have no effects on OpuA activity Lactococcus lactis
phosphatidylethanolamine non-bilayer lipid essential for high activity of OpuA Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
K3PO4 above 200 mM, inhibits ATPase activity Lactococcus lactis
Tetracaine low concentrations of the cationic amphipath decrease the activation of OpuA Lactococcus lactis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Lactococcus lactis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
K2SO4 100 mosmol/kg osmolality, stimulates OpuA activity Lactococcus lactis
K3PO4 100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity. 200 mM, stimulates ATPase activity Lactococcus lactis
KCl 100 mM, activates OpuA instantaneous and reversible Lactococcus lactis
Na2SO4 100 mosmol/kg osmolality, stimulates OpuA activity Lactococcus lactis
NaCl 100 mosmol/kg osmolality, stimulates OpuA activity Lactococcus lactis
Sodium phosphate 100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography of solubilized membranes Lactococcus lactis

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] = ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] the osmotic activation of the transporter is set by the bulk charge in the lipid headgroup region of the membrane, indicating that electrostatic interactions between lipids and the transporter are intrinsic to the osmosensing mechanism Lactococcus lactis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005
-
 ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg Lactococcus lactis
0.009
-
 ATPase activity, pH 7.0, 50 mM K3PO4, 290 mM sucrose, 450 mosmol/kg Lactococcus lactis
0.018
-
 ATPase activity, pH 7.0, 50 mM K3PO4, 90 mosmol/kg Lactococcus lactis
0.023
-
 ATPase activity, pH 7.0, 100 mM K3PO4, 180 mosmol/kg Lactococcus lactis
0.095
-
 ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM NaCl, 450 mosmol/kg Lactococcus lactis
0.099
-
 ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM KCl, 450 mosmol/kg Lactococcus lactis
0.117
-
 ATPase activity, pH 7.0, 150 mM K3PO4, 270 mosmol/kg Lactococcus lactis
0.124
-
 ATPase activity, pH 7.0, 350 mM K3PO4, 630 mosmol/kg Lactococcus lactis
0.146
-
 ATPase activity, pH 7.0, 250 mM K3PO4, 450 mosmol/kg Lactococcus lactis
0.148
-
 ATPase activity, pH 7.0, 50 mM K3PO4, 160 mM K2SO4, 450 mosmol/kg Lactococcus lactis
0.18
-
 ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + glycine betaine/out
-
 Lactococcus lactis ADP + phosphate + glycine betaine/in
-
 ?

Synonyms

Synonyms Comment Organism
OpuA
-
 Lactococcus lactis