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Literature summary for 7.6.2.8 extracted from

  • Borths, E.L.; Poolman, B.; Hvorup, R.N.; Locher, K.P.; Rees, D.C.
    In vitro functional characterization of BtuCD-F, the Escherichia coli ABC transporter for vitamin B12 uptake (2005), Biochemistry, 44, 16301-16309.
    View publication on PubMed
Search for more literature for 7.6.2.8

Activating Compound

Activating Compound Comment Organism Structure
periplasmic binding protein BtuF 0.02 mmol, 16% increase in basal activity in lauryl dimethylamine-N-oxide, 50% increase in activity in the presence of vitamin B12 in TX-100 Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) Escherichia coli

General Stability

General Stability Organism
BtuCD-F complex is stable in lauryl dimethylamine-N-oxide, dodecyl maltoside and Triton X-100 when storing the protein for several days at either 4°C or room temperature Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
periplasmic binding protein BtuF 4fold decrease of activity in n-alkyl-phosphocholine Escherichia coli
sodium ortho-vanadate
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-
periplasm
-
 Escherichia coli
-
-

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
NiNTA affinity chromatography, in the absence of vitamin B12 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 vitamin B12 has little effect on the activity in proteoliposomes, as well as in the presence of lauryl dimethylamine-N-oxide, dodecyl maltoside and Triton X-100 Escherichia coli
0.18
-
 in the absence of Btu-F and vitamin B12 in proteoliposomes Escherichia coli
0.33
-
 in the presence of Fos-choline Escherichia coli
0.44
-
 in the presence of 0.02 mmol BtuF in proteoliposomes Escherichia coli
0.98
-
 in the presence of lauryl dimethylamine-N-oxide Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + vitamin B12/out
-
 Escherichia coli ADP + phosphate + vitamin B12/in
-
 ?

Synonyms

Synonyms Comment Organism
BtuCD
-
 Escherichia coli
BtuCD-F complex of BtuCD and BtuF Escherichia coli