BRENDA - Enzyme Database show
show all sequences of 7.6.2.8

Identification of the btuCED polypeptides and evidence for their role in vitamin B12 transport in Escherichia coli

De Vaux, L.C.; Clevenson, D.S.; Bradbeer, C.; Kadner, R.J.; J. Bacteriol. 167, 920-927 (1986)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasmic membrane
btuC and btuD: associated
Escherichia coli
-
-
periplasm
btuE
Escherichia coli
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
btuE, nucleotide sequence
Escherichia coli
26000
-
btuC , nucleotide sequence
Escherichia coli
29000
-
btuD, nucleotide sequence
Escherichia coli
66000
-
btuB
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + H2O + vitamin B12/out
Escherichia coli
-
ADP + phosphate + vitamin B12/in
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + vitamin B12/out
-
210329
Escherichia coli
ADP + phosphate + vitamin B12/in
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
ABC-transporter
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasmic membrane
btuC and btuD: associated
Escherichia coli
-
-
periplasm
btuE
Escherichia coli
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
btuE, nucleotide sequence
Escherichia coli
26000
-
btuC , nucleotide sequence
Escherichia coli
29000
-
btuD, nucleotide sequence
Escherichia coli
66000
-
btuB
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + H2O + vitamin B12/out
Escherichia coli
-
ADP + phosphate + vitamin B12/in
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + vitamin B12/out
-
210329
Escherichia coli
ADP + phosphate + vitamin B12/in
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
ABC-transporter
Escherichia coli
Other publictions for EC 7.6.2.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749978
Agarwal
Mechanistic basis of vitamin ...
Vibrio cholerae serotype O1, Vibrio cholerae serotype O1 ATCC 39541, Vibrio cholerae serotype O1 Classical Ogawa 395, Vibrio cholerae serotype O1 O395
Biochim. Biophys. Acta
1867
140-151
2019
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1
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1
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8
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20
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1
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1
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1
1
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749821
Okamoto
Characterization of human ATP ...
Homo sapiens
Biochem. Biophys. Res. Commun.
496
1122-1127
2018
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1
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1
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2
2
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750412
Santos
Functional and structural cha ...
Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842, Lactobacillus delbrueckii subsp. bulgaricus DSM 20081, Lactobacillus delbrueckii subsp. bulgaricus JCM 1002, Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953, Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
eLife
7
e35828
2018
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1
1
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6
1
1
1
-
12
-
8
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18
1
1
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1
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1
1
1
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6
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1
1
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12
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18
1
1
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1
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2
2
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751644
Schmitt
Vitamin B12 import is all abo ...
Escherichia coli
Nat. Chem. Biol.
14
640-641
2018
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2
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751670
Rempel
Cysteine-mediated decyanation ...
Thiobacillus denitrificans
Nat. Commun.
9
3038
2018
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1
1
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1
1
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1
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1
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4
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4
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1
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1
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3
3
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751661
Goudsmits
Single-molecule visualization ...
Escherichia coli
Nat. Commun.
8
1652
2017
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1
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5
1
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3
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752215
Mireku
Structural basis of nanobody- ...
Escherichia coli
Sci. Rep.
7
14296
2017
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1
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3
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1
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1
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1
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752221
Mireku
Conformational change of a tr ...
Escherichia coli
Sci. Rep.
7
41575
2017
-
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1
1
8
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1
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2
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2
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1
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1
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3
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1
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1
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1
1
8
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1
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2
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1
1
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3
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1
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1
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3
3
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750063
Priess
Release of entropic spring re ...
Escherichia coli
Biophys. J.
110
2407-2418
2016
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1
1
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1
1
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2
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1
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1
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1
1
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2
2
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751956
Pan
ATP hydrolysis induced confor ...
Escherichia coli
PLoS ONE
11
e0166980
2016
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2
1
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1
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1
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752200
Kawaguchi
Translocation of the ABC tran ...
Homo sapiens
Sci. Rep.
6
30183
2016
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1
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1
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3
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1
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734003
Su
Conformational motions and fun ...
Escherichia coli
Int. J. Mol. Sci.
16
17933-17951
2015
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734281
Joseph
Conformational cycle of the vi ...
Escherichia coli
J. Biol. Chem.
289
3176-3185
2014
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1
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734760
Korkhov
Structure of AMP-PNP-bound Btu ...
Escherichia coli
Nat. Struct. Mol. Biol.
21
1097-1099
2014
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1
1
1
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734820
Gopinath
A vitamin B12 transporter in M ...
Mycobacterium tuberculosis
Open Biology
3
120175
2013
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719509
Korkhov
Asymmetric states of vitamin B ...
Escherichia coli
FEBS Lett.
586
972-976
2012
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1
1
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720849
Weng
-
The conformational transition ...
Escherichia coli
PLoS ONE
7
e305465
2012
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734771
Korkhov
Structure of AMP-PNP-bound vit ...
Escherichia coli
Nature
490
367-372
2012
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2
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719930
Di Bartolo
In vitro folding and assembly ...
Escherichia coli
J. Biol. Chem.
286
18807-18815
2011
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719987
Joseph
Transmembrane gate movements i ...
Escherichia coli
J. Biol. Chem.
286
41008-41017
2011
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713095
Lewinson
A distinct mechanism for the A ...
Escherichia coli
Nat. Struct. Mol. Biol.
17
332-338
2010
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713485
Kandt
Holo-BtuF stabilizes the open ...
Escherichia coli
Proteins
78
738-753
2010
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713505
Sun
Molecular dynamics simulation ...
Escherichia coli
Sci. China C Life Sci.
53
620-630
2010
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696776
Weng
Asymmetric conformational flex ...
Haemophilus influenzae
Biophys. J.
96
1918-1930
2009
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