Cloned (Comment) | Organism |
---|---|
gene ABCD1, recombinant expression of His-tagged protein ABCD1 in Pichia pastoris strain SMD1168 | Homo sapiens |
gene ABCD2, recombinant expression of protein ABCD2 in Pichia pastoris strain SMD1168 | Homo sapiens |
gene ABCD3, recombinant expression of protein ABCD3 in Pichia pastoris strain SMD1168 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AlF3 | moderate inhibition of the ATPase activity of liposome-reconstituted His-hABCD3; strong inhibition of the ATPase activity of liposome-reconstituted His-hABCD1; weak inhibition of the ATPase activity of liposome-reconstituted His-hABCD2 | Homo sapiens | |
additional information | no inhibition of the ATPase activity of liposome-reconstituted His-hABCD1 by NaN3, EGTA or ouabain | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisomal membrane | - |
Homo sapiens | 5778 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + fatty acyl CoA[side 1] | Homo sapiens | - |
ADP + phosphate + fatty acyl CoA[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P28288 | - |
- |
Homo sapiens | P33897 | - |
- |
Homo sapiens | Q9UBJ2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein ABCD1 from Pichia pastoris strain SMD1168 by detergent solubilization and nickel affinity chromatography, evaluation of diverse detergents | Homo sapiens |
recombinant His-tagged protein ABCD2 from Pichia pastoris strain SMD1168 by detergent solubilization and nickel affinity chromatography, evaluation of diverse detergents | Homo sapiens |
recombinant His-tagged protein ABCD3 from Pichia pastoris strain SMD1168 by detergent solubilization and nickel affinity chromatography, evaluation of diverse detergents | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
recombinant His-tagged protein ABCD1 protein solubilization and reconstitution in proteoliposomes | Homo sapiens |
recombinant His-tagged protein ABCD2 protein solubilization and reconstitution in proteoliposomes, the liposome-reconstituted His-hABCD1 shows ATPase activity | Homo sapiens |
recombinant His-tagged protein ABCD3 protein solubilization and reconstitution in proteoliposomes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + fatty acyl CoA[side 1] | - |
Homo sapiens | ADP + phosphate + fatty acyl CoA[side 2] | - |
? | |
ATP + H2O + NBD-palmitoyl-CoA[side 1] | - |
Homo sapiens | ADP + phosphate + NBD-palmitoyl-CoA[side 2] | - |
? | |
additional information | activity measurement by malachite green phosphate determination assay. Ellman's reagent, 5,5-dithiobis(2-nitrobenzoic acid), is not sensitive enough to measure the activity of hABCD1. The enzyme possesses an equal levels of acyl-CoA thioesterase activity | Homo sapiens | ? | - |
? | |
additional information | activity measurement by malachite green phosphate determination assay. Enzyme hABCD2 possesses acyl-CoA thioesterase activity | Homo sapiens | ? | - |
? | |
additional information | activity measurement by malachite green phosphate determination assay. Enzyme hABCD3 possesses acyl-CoA thioesterase activity | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, recombinant His-tagged ABCD3 | Homo sapiens |
? | x * 75000, recombinant His-tagged ABCD1 | Homo sapiens |
? | x * 75000, recombinant His-tagged ABCD2 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ABCD1 | - |
Homo sapiens |
ABCD2 | - |
Homo sapiens |
ABCD3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | ABCD1-3 belong to the ATP-binding cassette protein superfamily, subfamily D, membrane-bound proteins with highly conserved structure | Homo sapiens |