BRENDA - Enzyme Database
show all sequences of 7.6.2.4

Peroxisomal fatty acid uptake mechanism in Saccharomyces cerevisiae

van Roermund, C.W.; Ijlst, L.; Majczak, W.; Waterham, H.R.; Folkerts, H.; Wanders, R.J.; Hellingwerf, K.J.; J. Biol. Chem. 287, 20144-20153 (2012)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
peroxisome
;
Saccharomyces cerevisiae
5777
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
P34230
isoform Pxa2
-
Saccharomyces cerevisiae
P41909
isoform Pxa1
-
Saccharomyces cerevisiae ATCC 204508
P34230
isoform Pxa2
-
Saccharomyces cerevisiae ATCC 204508
P41909
isoform Pxa1
-
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
peroxisome
-
Saccharomyces cerevisiae
5777
-
General Information
General Information
Commentary
Organism
physiological function
very long chain acyl-CoA esters are hydrolyzed by the Pxa1p-Pxa2p complex prior to the actual transport of their fatty acid moiety into the peroxisomes with the CoA presumably being released into the cytoplasm. The Pxa1p-Pxa2p complex functionally interacts with the acyl-CoA synthetases Faa2p and/or Fat1p on the inner surface of the peroxisomal membrane for subsequent re-esterification of the very long chain fatty acids; very long chain acyl-CoA esters are hydrolyzed by the Pxa1p-Pxa2p complex prior to the actual transport of their fatty acid moiety into the peroxisomes with the CoA presumably being released into the cytoplasm. The Pxa1p-Pxa2p complex functionally interacts with the acyl-CoA synthetases Faa2p and/or Fat1p on the inner surface of the peroxisomal membrane for subsequent re-esterification of the very long chain fatty acids
Saccharomyces cerevisiae
General Information (protein specific)
General Information
Commentary
Organism
physiological function
very long chain acyl-CoA esters are hydrolyzed by the Pxa1p-Pxa2p complex prior to the actual transport of their fatty acid moiety into the peroxisomes with the CoA presumably being released into the cytoplasm. The Pxa1p-Pxa2p complex functionally interacts with the acyl-CoA synthetases Faa2p and/or Fat1p on the inner surface of the peroxisomal membrane for subsequent re-esterification of the very long chain fatty acids
Saccharomyces cerevisiae
Other publictions for EC 7.6.2.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750514
Violante
Peroxisomes can oxidize mediu ...
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1
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4
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1
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7
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7
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2
2
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749821
Okamoto
Characterization of human ATP ...
Homo sapiens
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496
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1
1
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3
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9
1
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3
3
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4
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3
3
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3
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3
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9
3
3
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3
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1
3
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750860
Andreoletti
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5
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Geillon
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8
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6
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van Roermund
Peroxisomal fatty acid uptake ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
J. Biol. Chem.
287
20144-20153
2012
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2
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720023
Hillebrand
Identification of a new fatty ...
Homo sapiens
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2012
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1
1
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718980
van Roermund
Differential substrate specifi ...
Homo sapiens
Biochim. Biophys. Acta
1811
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2011
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1
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2
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1
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Boisnard
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Podospora anserina
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1
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Rattus norvegicus
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1
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696484
Leclercq
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Rattus norvegicus
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1
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697851
van Roermund
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Saccharomyces cerevisiae, Saccharomyces cerevisiae BJ1991
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2008
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1
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2
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7
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2
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2
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687606
Hillebrand
Live cell FRET microscopy: hom ...
Homo sapiens
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2007
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1
1
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5
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2
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1
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1
1
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1
1
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688498
Takahashi
Adrenoleukodystrophy: subcellu ...
Homo sapiens
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101
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2007
-
1
1
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9
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1
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1
1
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2
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1
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1
1
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9
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689002
Kemp
X-linked adrenoleukodystrophy: ...
Mammalia, Saccharomyces cerevisiae
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90
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2007
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1
1
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667429
Gueugnon
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341
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1
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667893
Morita
ATP-binding and -hydrolysis ac ...
Rattus norvegicus
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2006
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1
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1
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5
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1
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1
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667804
Kashiwayama
Role of Pex19p in the targetin ...
Homo sapiens
Biochim. Biophys. Acta
1746
116-128
2005
1
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656347
Van Roermund
The peroxisomal lumen in Sacch ...
Saccharomyces cerevisiae
J. Cell Sci.
117
4231-4237
2004
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667776
Guimaraes
Mouse liver PMP70 and ALDP: ho ...
Mus musculus
Biochim. Biophys. Acta
1689
235-243
2004
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656975
Hayashi
Ped3 is a peroxisomal ATP-bind ...
Arabidopsis thaliana
Plant Cell Physiol.
43
1-11
2002
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1
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1
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210404
Hettema
Transport of fatty acids and m ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1486
18-27
2000
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210408
Imanaka
Characterization of the 70-kDa ...
Rattus norvegicus
J. Biol. Chem.
274
11968-11976
1999
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1
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3
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210409
Liu
Homo- and heterodimerization o ...
Homo sapiens, Mus musculus
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1999
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210410
Netik
Adrenoleukodystrophy-related p ...
Homo sapiens, Mus musculus
Hum. Mol. Genet.
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907-913
1999
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210406
Gartner
Genomic organization of the 70 ...
Homo sapiens
Genomics
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1998
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210412
Verleur
Transport of activated fatty a ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae Pxa2p
Eur. J. Biochem.
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657-661
1997
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6
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210405
Contreras
Topology of ATP-binding domain ...
Rattus norvegicus
Arch. Biochem. Biophys.
334
369-379
1996
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210407
Hettema
The ABC transporter proteins P ...
Saccharomyces cerevisiae
EMBO J.
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3813-3822
1996
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210411
Shani
A Saccharomyces cerevisiae hom ...
Homo sapiens, Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
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11901-11906
1996
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210403
Kamijo
The 70-kDa peroxisomal membran ...
Rattus norvegicus
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4534-4540
1990
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