Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.6.2.4 extracted from

  • Morita, M.; Kurisu, M.; Kashiwayama, Y.; Yokota, S.; Imanaka, T.
    ATP-binding and -hydrolysis activities of ALDP (ABCD1) and ALDRP (ABCD2), human peroxisomal ABC proteins, overexpressed in Sf21 cells (2006), Biol. Pharm. Bull., 29, 1836-1842.
    View publication on PubMed
Search for more literature for 7.6.2.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Spodoptera frugiperda strain 21 cells Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information the ATPase activity of ALDP-His is insensitive to oligomycin, azide and vanadate Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-
nucleus
-
 Rattus norvegicus 5634
-
peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 ALDP-His, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
ALDP-His, immobilized metal affinity chromatography Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + fatty acyl CoA[side 1] = ADP + phosphate + fatty acyl CoA[side 2] ALDP exhibits an affinity to both ADP and ATP, ALDRP exhibits an affinity to ADP but scarcely to ATP Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0743
-
 ALDRP-His, membrane fractions (about 10 mg of membrane protein) are incubated at 37°C in 0.1 ml of 40 mM Tris-HCl (pH 7.4) containing 0.1 mM EGTA, 1 mM dithiothreitol, 2mM ouabain and 15 mM MgSO4, and 5 mM NaATP Rattus norvegicus
0.0774
-
 ALDP-His, membrane fractions (about 10 mg of membrane protein) are incubated at 37°C in 0.1 ml of 40 mM Tris-HCl (pH 7.4) containing 0.1 mM EGTA, 1 mM dithiothreitol, 2mM ouabain and 15 mM MgSO4, and 5 mM NaATP Rattus norvegicus

Synonyms

Synonyms Comment Organism
adrenoleukodystrophy protein ALDP, ABCD Rattus norvegicus
ALD-related protein ALDRP, ABCD2 Rattus norvegicus