Literature summary for 7.6.2.3 extracted from

Rebbeor, J.; Connolly, G.; Henson, J.; Boyer, J.; Ballatori, N.
ATP-dependent GSH and glutathione S-conjugate transport in skate liver Role of an Mrp functional homologue (2000), Am. J. Physiol., 279, G417-G425 .

Search for more literature for 7.6.2.3

Activating Compound

Activating Compound	Comment	Organism	Structure
digoxin	103% activity at 0.1 mM	Leucoraja erinacea
N-7[(4-nitrobenzo-2-oxa-1,3-diazol)]-7beta-amino-3alpha,12alpha-dihydroxy-5beta-cholan-24-oyl-2-aminoethanesulfonate	110% activity at 0.l mM	Leucoraja erinacea
p-aminohippuric acid	107% activity at 0.1 mM	Leucoraja erinacea
taurocholate	103% activity at 0.1 mM	Leucoraja erinacea

Inhibitors

Inhibitors	Comment	Organism	Structure
4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid	10% residual activity at 1 mM	Leucoraja erinacea
gramicidin D	97% residual activity at 0.01 mM	Leucoraja erinacea
GSH	73% residual activity at 20 mM	Leucoraja erinacea
GSSG	28% residual activity at 0.1 mM	Leucoraja erinacea
leukotriene C4	9% residual activity at 0.1 mM	Leucoraja erinacea
S-(2,4-dinitrophenyl)glutathione	64% residual activity at 0.0007 mM	Leucoraja erinacea
Sulfobromophthalein	33% residual activity at 0.1 mM	Leucoraja erinacea
vanadate	32% residual activity at 0.1 mM	Leucoraja erinacea
verapamil	87% residual activity at 0.01 mM	Leucoraja erinacea
vincristine	85% residual activity at 0.01 mM	Leucoraja erinacea

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.032	-	S-(2,4-dinitrophenyl)glutathione[side 1]	at pH 7.5 and 20°C	Leucoraja erinacea
0.4	-	ATP	with S-(2,4-dinitrophenyl)glutathione as cosubstrate, at pH 7.5 and 20°C	Leucoraja erinacea
0.5	-	ATP	with S-glutathione as cosubstrate, at pH 7.5 and 20°C	Leucoraja erinacea
12	-	S-glutathione[side 1]	at pH 7.5 and 20°C	Leucoraja erinacea

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	-	Leucoraja erinacea	5886	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required in form of Mg-ATP	Leucoraja erinacea

Organism

Organism	UniProt	Comment	Textmining
Leucoraja erinacea	Q8QG98	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
hepatocyte	-	Leucoraja erinacea	-
liver	-	Leucoraja erinacea	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + S-(2,4-dinitrophenyl)glutathione[side 1]	-	Leucoraja erinacea	ADP + phosphate + S-(2,4-dinitrophenyl)glutathione[side 2]	-	?
ATP + H2O + S-ethylglutathione[side 1]	-	Leucoraja erinacea	ADP + phosphate + S-ethylglutathione[side 2]	-	?
ATP + H2O + S-glutathione[side 1]	-	Leucoraja erinacea	ADP + phosphate + S-glutathione[side 2]	-	?
additional information	no activity with adenylyl-imidodiphosphate	Leucoraja erinacea	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 180000, SDS-PAGE	Leucoraja erinacea

Synonyms

Synonyms	Comment	Organism
MRP2	-	Leucoraja erinacea

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Leucoraja erinacea

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Release 2023.1 (January 2023)