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Literature summary for 7.6.2.2 extracted from

  • Rahman, S.J.; Kaur, P.
    Conformational changes in a multidrug resistance ABC transporter DrrAB Fluorescence-based approaches to study substrate binding (2018), Arch. Biochem. Biophys., 658, 31-45 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli HMS174(DELTADE3) cells Streptomyces peucetius

Protein Variants

Protein Variants Comment Organism
E165Q the mutation shows 3% doxorubicin efflux compared to the wild type enzyme Streptomyces peucetius
K47M the mutation exhibits no doxorubicin efflux compared to the wild type enzyme Streptomyces peucetius
K47R the mutation shows 24% doxorubicin efflux compared to the wild type enzyme Streptomyces peucetius
Q197H the mutation shows 15% doxorubicin efflux compared to the wild type enzyme Streptomyces peucetius
S23C the mutation has a slight decreasing effect on doxorubicin efflux compared to the wild type enzyme Streptomyces peucetius
S319C the mutation has a slight decreasing effect on doxorubicin efflux compared to the wild type enzyme Streptomyces peucetius
Y18A the mutation exhibits no doxorubicin efflux compared to the wild type enzyme Streptomyces peucetius
Y89C the mutation has no effect on doxorubicin efflux compared to the wild type enzyme Streptomyces peucetius

Organism

Organism UniProt Comment Textmining
Streptomyces peucetius
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + doxorubicin[side 1]
-
 Streptomyces peucetius ADP + phosphate + doxorubicin[side 2]
-
 ?
ATP + H2O + ethidium bromide[side 1]
-
 Streptomyces peucetius ADP + phosphate + ethidium bromide[side 2]
-
 ?
ATP + H2O + Hoechst33342[side 1]
-
 Streptomyces peucetius ADP + phosphate + Hoechst33342[side 2]
-
 ?
ATP + H2O + rhodamine B[side 1]
-
 Streptomyces peucetius ADP + phosphate + rhodamine B[side 2]
-
 ?
ATP + H2O + verapamil[side 1]
-
 Streptomyces peucetius ADP + phosphate + verapamil[side 2]
-
 ?
ATP + H2O + vinblastine[side 1]
-
 Streptomyces peucetius ADP + phosphate + vinblastine[side 2]
-
 ?
additional information subunit DrrA of DrrAB hydrolyzes ATP, and the energy is transduced to carrier DrrB resulting in export of drugs Streptomyces peucetius ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 35500 + 1 * 30600, SDS-PAGE Streptomyces peucetius

Synonyms

Synonyms Comment Organism
DrrAB
-
 Streptomyces peucetius
multidrug resistance ABC transporter
-
 Streptomyces peucetius