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Literature summary for 7.6.2.2 extracted from

  • Jin, M.S.; Oldham, M.L.; Zhang, Q.; Chen, J.
    Crystal structure of the multidrug transporter P-glycoprotein from Caenorhabditis elegans (2012), Nature, 490, 566-569.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
actinomycin D 1 mM increases the ATPase activity of the enzyme more than 5times Caenorhabditis elegans
dipyridamole 1 mM increases the ATPase activity of the enzyme more than 5times Caenorhabditis elegans
paclitaxel 1 mM increases the ATPase activity of the enzyme more than 5times Caenorhabditis elegans
progesterone 1 mM increases the ATPase activity of the enzyme more than 5times Caenorhabditis elegans
valinomycin 1 mM increases the ATPase activity of the enzyme more than 5times Caenorhabditis elegans

Cloned(Commentary)

Cloned (Comment) Organism
native protein is expressed in Pichia pastoris strain SMD1163 and Sf9 insect cells, while selenomethionine-labeled protein is expressed in Hi5 cells Caenorhabditis elegans

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, native protein is crystallized by using 100 mM HEPES pH 6.6-7.2, 200 mM sodium malonate and 19-22% (w/v) PEG 2000-MME, at 4°C. Crystals of selenomethionine-substituted protein are obtained with 100 mM HEPES pH 6.6-7.2, 200 mM ammonium phosphate monobasic and 19-22% PEG 1500 at 4°C Caenorhabditis elegans

Inhibitors

Inhibitors Comment Organism Structure
vanadate complete inhibition at 1 mM Caenorhabditis elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Caenorhabditis elegans 16020
-

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans P34712
-
-

Purification (Commentary)

Purification (Comment) Organism
cobalt affinity resin column chromatography, GST column chromatography, and Superdex 200 gel filtration Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + actinomycin D/in
-
Caenorhabditis elegans ADP + phosphate + actinomycin D/out
-
?
ATP + H2O + paclitaxel/in
-
Caenorhabditis elegans ADP + phosphate + paclitaxel/out
-
?

Synonyms

Synonyms Comment Organism
P-glycoprotein
-
Caenorhabditis elegans
P-gp
-
Caenorhabditis elegans