Literature summary for 7.6.2.2 extracted from

Sauna, Z.E.; Nandigama, K.; Ambudkar, S.V.
Exploiting reaction intermediates of the ATPase reaction to elucidate the mechanism of transport by P-glycoprotein (ABCB1) (2006), J. Biol. Chem., 281, 26501-26511.

Search for more literature for 7.6.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in insect cell	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E556Q/E1201Q	Walker B mutant, traps nucleotide in the absence of vandate or beryllium fluoride. Use for identification of reaction intermediates	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isoform MDR1	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + H2O + xenobiotic[side 1] = ADP + phosphate + xenobiotic[side 2]	the high to low affinity switch in the transport substrate binding site is due to the formation of the E-S reaction intermediate of the ATPase reaction. The posthydrolysis E-P state continues to have low affinity for substrate, suggesting that conformational changes that form the E-S complex are coupled to the conformational change at the transport substrate site to do mechanical work. The formation of E-S reaction intermediate during a single turnover of the catalytic cycle appears to provide the initial power stroke for movement of drug substrate from inner leaflet to outer leaflet of lipid bilayer	Homo sapiens	a

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Release 2023.1 (January 2023)