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Literature summary for 7.6.2.2 extracted from

  • Carrier, I.; Urbatsch, I.L.; Senior, A.E.; Gros, P.
    Mutational analysis of conserved aromatic residues in the A-loop of the ABC transporter ABCB1A (mouse Mdr3) (2007), FEBS Lett., 581, 301-308.
    View publication on PubMed
Search for more literature for 7.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Mus musculus

Protein Variants

Protein Variants Comment Organism
Y1040W greatly reduced ATPase activity, impaired transport Mus musculus
Y1263W catalytic parameters similar to wild-type Mus musculus
Y397W greatly reduced ATPase activity, impaired transport Mus musculus
Y618W catalytic parameters similar to wild-type Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
FK506
-
 Mus musculus
valinomycin
-
 Mus musculus
verapamil
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 ATP pH 8.0, 37°C, wild-type Mus musculus
0.4
-
 ATP pH 8.0, 37°C, wild-type, presence of valinomycin Mus musculus
0.5
-
 ATP pH 8.0, 37°C, wild-type, presence of FK506 Mus musculus
0.5
-
 ATP pH 8.0, 37°C, wild-type, presence of verapamil Mus musculus
1.5
-
 ATP pH 8.0, 37°C, mutant Y1040W Mus musculus
2.6
-
 ATP pH 8.0, 37°C, mutant Y1040W, presence of verapamil Mus musculus
3
-
 ATP pH 8.0, 37°C, mutant Y1040W, presence of valinomycin Mus musculus
3.5
-
 ATP pH 8.0, 37°C, mutant Y397W, presence of verapamil Mus musculus
5.1
-
 ATP pH 8.0, 37°C, mutant Y397W, presence of valinomycin Mus musculus
7.1
-
 ATP pH 8.0, 37°C, mutant Y1040W, presence of FK506 Mus musculus
8.4
-
 ATP pH 8.0, 37°C, mutant Y397W, presence of FK506 Mus musculus
9
-
 ATP pH 8.0, 37°C, mutant Y397W Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 isoform Mdr3, i.e. ABCB1A
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Mus musculus ADP + phosphate
-
 ?