Literature summary for 7.6.2.2 extracted from

Tombline, G.; Urbatsch, I.L.; Virk, N.; Muharemagic, A.; White, L.B.; Senior, A.E.
Expression, purification, and characterization of cysteine-free mouse P-glycoprotein (2006), Arch. Biochem. Biophys., 445, 124-128.

Search for more literature for 7.6.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
additional information	cysteine-free mouse MDR3 P-glycoprotein (Pgp) is constructed by mutagenesis of the nine natural Cys to Ala. Yield, purity, ATPase activity, KM (MgATP), and stimulation of ATPase by verapamil are similar to wild-type mouse Ppg. After long-term storage at -70 °C (6 months), or incubation at 37 °C for up to 2h, it shows the same stability as wild type (as measured by retention of ATPase activity)	Mus musculus
Y1040A	very low ATPase activity	Mus musculus
Y1040C	very low ATPase activity	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.21	-	ATP	wild-type enzyme	Mus musculus
0.22	-	ATP	Cys-free mutant enzyme	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
cysteine-free mouse MDR3 P-glycoprotein (Pgp) is constructed by mutagenesis of the nine natural Cys to Ala, expressed in Pichia pastoris and purified	Mus musculus

Storage Stability

Storage Stability	Organism
-70°C, 6 months, cysteine-free mutant shows the same stability as the wild-type enzyme	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + verapamil/in	-	Mus musculus	ADP + phosphate + verapamil/out	-	?

Synonyms

Synonyms	Comment	Organism
MDR3 P-glycoprotein	-	Mus musculus

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Release 2023.1 (January 2023)