Protein Variants | Comment | Organism |
---|---|---|
E504A | mutant is fully devoid of ATPase activity | Bacillus subtilis |
E504C | mutant is fully devoid of ATPase activity | Bacillus subtilis |
E504D | mutant is fully devoid of ATPase activity | Bacillus subtilis |
E504Q | mutant is fully devoid of ATPase activity | Bacillus subtilis |
E504S | mutant is fully devoid of ATPase activity | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
vanadate | - |
Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bacillus subtilis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+-dependence of 8-aza-ATP binding to wild-type and mutant BmrA | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus subtilis | BmrA is a half-ABC transporter involved in multidrug resistance | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant BmrA | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
pH 8, 37°C, 8-aza-ATPase activity | Bacillus subtilis |
6.5 | - |
pH 8, 37°C, ATPase activity | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-aza-ATP + H2O + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/in | 8-aza-ATP is a fairly good substrate of BmrA | Bacillus subtilis | 8-aza-ADP + phosphate + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/out | - |
? | |
ATP + H2O + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/in | Hoechst 33342, half-ABC transporter BmrA, Glu-504, adjacent to the Walker-B motif, is the catalytic base for ATP hydrolysis | Bacillus subtilis | ADP + phosphate + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/out | - |
? | |
additional information | BmrA is a half-ABC transporter involved in multidrug resistance | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BmrA | formerly known as YvcC | Bacillus subtilis |
YvcC | renamed BmrA | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus subtilis |