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Literature summary for 7.6.2.2 extracted from

  • Steinfels, E.; Orelle, C.; Dalmas, O.; Penin, F.; Miroux, B.; Di Pietro, A.; Jault, J.M.
    Highly efficient over-production in E. coli of YvcC, a multidrug-like ATP-binding cassette transporter from Bacillus subtilis (2002), Biochim. Biophys. Acta, 1565, 1-5.
    View publication on PubMed
Search for more literature for 7.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
yvcC gene, overexpression in Escherichia coli C41(DE3) Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
vanadate ATPase activity is highly sensitive to vanadate inhibition, 0.01 mM: 50% inhibition Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane recombinant YvcC is highly enriched in membrane vesicles and embedded in the membrane in a fully functional conformation Bacillus subtilis 16020
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Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + xenobiotic[side 1] = ADP + phosphate + xenobiotic[side 2] ABC-type, ATP-binding cassette-type, ATPase, characterized by the presence of two similar ATP-binding domains, does not undergo phosphorylation during the transport process, enzymes of Gram-positive bacteria and eukaryotic cells that export a number of drugs, with unusual specificity, covering various groups of unrelated substances, while ignoring some that are closely related structurally, several distinct enzymes may be present in a single eukaryotic cell, many of them transport glutathione conjugates with drugs, some also show some flippase, Mg2+-ATPase, EC 3.6.3.1, activity Bacillus subtilis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/in Hoechst 33342, ABC transporter YvcC Bacillus subtilis ADP + phosphate + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/out
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 ?

Synonyms

Synonyms Comment Organism
YvcC
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 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
 assay at, ATPase activity Bacillus subtilis
25
-
 assay at, Hoechst 33342 transport Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bacillus subtilis