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Literature summary for 7.6.2.16 extracted from

  • Terui, Y.; Saroj, S.D.; Sakamoto, A.; Yoshida, T.; Higashi, K.; Kurihara, S.; Suzuki, H.; Toida, T.; Kashiwagi, K.; Igarashi, K.
    Properties of putrescine uptake by PotFGHI and PuuP and their physiological significance in Escherichia coli (2014), Amino Acids, 46, 661-670 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli polyamine-deficient KK3131 cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
putrescine putrescine uptake by the enzyme is gradually inhibited by increasing levels of putrescine Escherichia coli
spermidine putrescine uptake by the enzyme is gradually inhibited by increasing levels of spermidine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00057
-
putrescine-[putrescine-binding protein][side 1] pH and temperature not specified in the publication Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + putrescine-[putrescine-binding protein][side 1] Escherichia coli
-
ADP + phosphate + putrescine[side 2] + [putrescine-binding protein][side 1]
-
?
ATP + H2O + putrescine-[putrescine-binding protein][side 1] Escherichia coli W3110
-
ADP + phosphate + putrescine[side 2] + [putrescine-binding protein][side 1]
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli W3110
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + putrescine-[putrescine-binding protein][side 1]
-
Escherichia coli ADP + phosphate + putrescine[side 2] + [putrescine-binding protein][side 1]
-
?
ATP + H2O + putrescine-[putrescine-binding protein][side 1]
-
Escherichia coli W3110 ADP + phosphate + putrescine[side 2] + [putrescine-binding protein][side 1]
-
?

Synonyms

Synonyms Comment Organism
potFGHI
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Escherichia coli