Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.6.2.11 extracted from

  • Igarashi, K.; Ito, K.; Kashiwagi, K.
    Polyamine uptake systems in Escherichia coli (2001), Res. Microbiol., 152, 271-278.
    View publication on PubMed
Search for more literature for 7.6.2.11

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of the potD subunit and a potF - putrescine complex Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 spermidine/out spermidine-preferential system Escherichia coli
0.0015
-
 putrescine/out spermidine-preferential system Escherichia coli
0.005
-
 putrescine/out putrescine-specific system Escherichia coli
0.385
-
 ATP ATPase activity of the potA subunit in the spermidine-preferential system Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 ? * 43000 + ? * 31000 + ? * 29000 + ? * 39000, potA, potB, potC and potD subunits respectively, spermidine-preferential system, calculated from amino acid sequence Escherichia coli
31000
-
 ? * 38000 + ? * 45000 + ? * 31000 + ? * 35000, potF, potG, potH and potI subunits respectively, putrescine-specific system, calculated from amino acid sequence Escherichia coli
31000
-
 ? * 43000 + ? * 31000 + ? * 29000 + ? * 39000, potA, potB, potC and potD subunits respectively, spermidine-preferential system, calculated from amino acid sequence Escherichia coli
35000
-
 ? * 38000 + ? * 45000 + ? * 31000 + ? * 35000, potF, potG, potH and potI subunits respectively, putrescine-specific system, calculated from amino acid sequence Escherichia coli
38000
-
 ? * 38000 + ? * 45000 + ? * 31000 + ? * 35000, potF, potG, potH and potI subunits respectively, putrescine-specific system, calculated from amino acid sequence Escherichia coli
39000
-
 ? * 43000 + ? * 31000 + ? * 29000 + ? * 39000, potA, potB, potC and potD subunits respectively, spermidine-preferential system, calculated from amino acid sequence Escherichia coli
43000
-
 ? * 43000 + ? * 31000 + ? * 29000 + ? * 39000, potA, potB, potC and potD subunits respectively, spermidine-preferential system, calculated from amino acid sequence Escherichia coli
45000
-
 ? * 38000 + ? * 45000 + ? * 31000 + ? * 35000, potF, potG, potH and potI subunits respectively, putrescine-specific system, calculated from amino acid sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
 ATPase activity of the potA subunit in the spermidine-preferential system Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + putrescine/out putrescine-ornithine antiporter, encoded by the gene potE Escherichia coli ADP + phosphate + putrescine/in
-
 r
ATP + H2O + putrescine/out the putrescine-specific system, encoded by the genes potABCD, is specific for this polyamine Escherichia coli ADP + phosphate + putrescine/in
-
 ?
ATP + H2O + spermidine/out the spermidine-preferential system, encoded by the genes potFGHI, can transport both spermidine and putrescine Escherichia coli ADP + phosphate + spermidine/in
-
 ?

Subunits

Subunits Comment Organism
oligomer ? * 38000 + ? * 45000 + ? * 31000 + ? * 35000, potF, potG, potH and potI subunits respectively, putrescine-specific system, calculated from amino acid sequence Escherichia coli
oligomer ? * 43000 + ? * 31000 + ? * 29000 + ? * 39000, potA, potB, potC and potD subunits respectively, spermidine-preferential system, calculated from amino acid sequence Escherichia coli