Any feedback?
Literature summary for 7.6.2.10 extracted from
- Oxygen limitation enhances the antimicrobial activity of fosfomycin in Pseudomonas aeruginosa following overexpression of glpT which encodes glycerol-3-phosphate/fosfomycin symporter (2018), Front. Microbiol., 9, 1950 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene glpT, sequence comparisons with ANR binding site and glpT transcriptional start site, construction of lacZ reporter plasmids to measure promoter activities of glpT in PAO1 and Ps.a-682, and fosA and glpR in PAO1, designated pBBRglpT(PAO1)-P, pBBRglpT(Ps.a-682)-P, pBBRfosA(PAO1)-P, and pBBRglpR(PAO1)-P, respectively | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an in-frame deletion of glpT in PAO1 background, PAO1DELTAglpT | Pseudomonas aeruginosa |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Pseudomonas aeruginosa |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | Pseudomonas aeruginosa | - |
ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | Pseudomonas aeruginosa ATCC 15692 | - |
ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | Pseudomonas aeruginosa 1C | - |
ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | Pseudomonas aeruginosa PRS 101 | - |
ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | Pseudomonas aeruginosa DSM 22644 | - |
ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | Pseudomonas aeruginosa CIP 104116 | - |
ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | Pseudomonas aeruginosa LMG 12228 | - |
ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | Pseudomonas aeruginosa JCM 14847 | - |
ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| additional information | Pseudomonas aeruginosa | the GlpT transporter also transports fosfomycin | ? | - |
? | |
| additional information | Pseudomonas aeruginosa ATCC 15692 | the GlpT transporter also transports fosfomycin | ? | - |
? | |
| additional information | Pseudomonas aeruginosa 1C | the GlpT transporter also transports fosfomycin | ? | - |
? | |
| additional information | Pseudomonas aeruginosa PRS 101 | the GlpT transporter also transports fosfomycin | ? | - |
? | |
| additional information | Pseudomonas aeruginosa DSM 22644 | the GlpT transporter also transports fosfomycin | ? | - |
? | |
| additional information | Pseudomonas aeruginosa CIP 104116 | the GlpT transporter also transports fosfomycin | ? | - |
? | |
| additional information | Pseudomonas aeruginosa LMG 12228 | the GlpT transporter also transports fosfomycin | ? | - |
? | |
| additional information | Pseudomonas aeruginosa JCM 14847 | the GlpT transporter also transports fosfomycin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
Ps.a-682 clinical isolate from sputum | - |
| Pseudomonas aeruginosa | Q9HTV5 | - |
- |
| Pseudomonas aeruginosa 1C | Q9HTV5 | - |
- |
| Pseudomonas aeruginosa ATCC 15692 | Q9HTV5 | - |
- |
| Pseudomonas aeruginosa CIP 104116 | Q9HTV5 | - |
- |
| Pseudomonas aeruginosa DSM 22644 | Q9HTV5 | - |
- |
| Pseudomonas aeruginosa JCM 14847 | Q9HTV5 | - |
- |
| Pseudomonas aeruginosa LMG 12228 | Q9HTV5 | - |
- |
| Pseudomonas aeruginosa PRS 101 | Q9HTV5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | - |
Pseudomonas aeruginosa | ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | - |
Pseudomonas aeruginosa ATCC 15692 | ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | - |
Pseudomonas aeruginosa 1C | ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | - |
Pseudomonas aeruginosa PRS 101 | ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | - |
Pseudomonas aeruginosa DSM 22644 | ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | - |
Pseudomonas aeruginosa CIP 104116 | ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | - |
Pseudomonas aeruginosa LMG 12228 | ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| ATP + H2O + sn-glycerol 3-phosphate-[glycerol 3-phosphate-binding protein][side 1] | - |
Pseudomonas aeruginosa JCM 14847 | ADP + phosphate + sn-glycerol 3-phosphate[side 2] + [glycerol 3-phosphate-binding protein][side 1] | - |
? | |
| additional information | the GlpT transporter also transports fosfomycin | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | the GlpT transporter also transports fosfomycin | Pseudomonas aeruginosa ATCC 15692 | ? | - |
? | |
| additional information | the GlpT transporter also transports fosfomycin | Pseudomonas aeruginosa 1C | ? | - |
? | |
| additional information | the GlpT transporter also transports fosfomycin | Pseudomonas aeruginosa PRS 101 | ? | - |
? | |
| additional information | the GlpT transporter also transports fosfomycin | Pseudomonas aeruginosa DSM 22644 | ? | - |
? | |
| additional information | the GlpT transporter also transports fosfomycin | Pseudomonas aeruginosa CIP 104116 | ? | - |
? | |
| additional information | the GlpT transporter also transports fosfomycin | Pseudomonas aeruginosa LMG 12228 | ? | - |
? | |
| additional information | the GlpT transporter also transports fosfomycin | Pseudomonas aeruginosa JCM 14847 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlpT | - |
Pseudomonas aeruginosa |
| glycerol-3-phosphate transporter | - |
Pseudomonas aeruginosa |
| glycerol-3-phosphate/fosfomycin symporter | - |
Pseudomonas aeruginosa |
| PA5235 | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Pseudomonas aeruginosa | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Pseudomonas aeruginosa | GlpR is a repressor for the glpT gene expression, no significant difference in glpR expression between both conditions, aerobic and anaerobic growth | down |
| Pseudomonas aeruginosa | oxygen limitation leads to overexpression in Pseudomonas aeruginosa. Anaerobic regulator protein ANR is an activator of glpT gene expression, it binds to the DNA fragment from glpT region upstream | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | oxygen limitation enhances the antimicrobial activity of fosfomycin in Pseudomonas aeruginosa following overexpression of glpT which encodes glycerol-3-phosphate/fosfomycin symporter. Glycerol-3-phosphate is a native substrate of GlpT transporter, and it is a sole carbon source for growth. Cells grown anaerobically exhibit a higher expression of glpT encoding a glycerol-3-phosphate transporter which is responsible for fosfomycin uptake, causing increased intracellular accumulation of the drug. Increased GlpT expression is due to activation by ANR, resulting in increased uptake of the drug fosfomycin leading to increased antibacterial activity. GlpT is only the transporter for fosfomycin uptake in Pseudomonas aeruginosa since it lacks the uhpT gene. Apart from GlpT, Pseudomonas aeruginosa produces chromosomally encoded FosA that confers innate tolerance by inactivating fosfomycin. Fosfomycin is more active under anaerobic conditions to not only strain PAO1 but also the clinical isolate Ps.a-682 | Pseudomonas aeruginosa |
| physiological function | oxygen limitation enhances the antimicrobial activity of fosfomycin in Pseudomonas aeruginosa following overexpression of glpT which encodes glycerol-3-phosphate/fosfomycin symporter. The clinical isolate Ps.a-682 exhibits a promoted biofilm phenotype. Glycerol-3-phosphate is a native substrate of GlpT transporter, and it is a sole carbon source for growth. Cells grown anaerobically exhibit a higher expression of glpT encoding a glycerol-3-phosphate transporter which is responsible for fosfomycin uptake, causing increased intracellular accumulation of the drug. Increased GlpT expression is due to activation by ANR, resulting in increased uptake of the drug fosfomycin leading to increased antibacterial activity. GlpT is only the transporter for fosfomycin uptake in Pseudomonas aeruginosa since it lacks the uhpT gene. Apart from GlpT, Pseudomonas aeruginosa produces chromosomally encoded FosA that confers innate tolerance by inactivating fosfomycin. Fosfomycin is more active under anaerobic conditions to not only strain PAO1 but also the clinical isolate Ps.a-682 | Pseudomonas aeruginosa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
