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Literature summary for 7.6.2.10 extracted from

  • Santoro, A.; Cappello, A.R.; Madeo, M.; Martello, E.; Iacopetta, D.; Dolce, V.
    Interaction of fosfomycin with the glycerol 3-phosphate transporter of Escherichia coli (2011), Biochim. Biophys. Acta, 1810, 1323-1329.
    View publication on PubMed
Search for more literature for 7.6.2.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme in Escherichia coli strain SH116 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 40.5% inhibition at 0.1 mM Escherichia coli
bathophenanthroline 96.2% inhibition at 10 mM Escherichia coli
fosfomycin fosfomycin, externally added to the proteoliposomes, poorly inhibits the glycerol-3-phosphate/glycerol-3-phosphate antiport catalyzed by the reconstituted transporter, completely competitive, interaction of fosfomycin with the recombinant and purified GlpT of Escherichia coli reconstituted in liposomes, overview Escherichia coli
Mersalyl 71.1% inhibition at 0.1 mM Escherichia coli
N-ethylmaleimide 1.5% inhibition at 1 mM Escherichia coli
Phthalic acid 48.8% inhibition at 10 mM Escherichia coli
pyridoxal 5'-phosphate 98.5% inhibition at 10 mM Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 55fold from Escherichia coli strain SH116 by nickel affinity chromatography Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of the recombinant His-tagged GlpT into liposomes Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme performs G3P/G3P exchange, it shows virtually poor exchange activity using phosphoenolpyruvate, fosfomycin and sulphate, and no activity with glutamate, glutamine, aspartate and ATP. Highest activity in the presence of internal glycerol-3-phosphate and phosphate Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
GlpT
-
 Escherichia coli
glycerol 3-phosphate transporter
-
 Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.4
-
 externally added to the proteoliposomes with reconstituted transporter, pH and temperature not specified in the publication Escherichia coli fosfomycin

General Information

General Information Comment Organism
additional information fosfomycin is widely used to treat urinary tract and pediatric gastrointestinal infections of bacteria. It is supposed that this antibiotic enters cells via two transport systems, including the bacterial glycerol-3-phosphate transporter, GlpT. Impaired function of GlpT is one mechanism for fosfomycin resistance. Interaction of fosfomycin with the recombinant and purified GlpT of Escherichia coli reconstituted in liposomes, overview Escherichia coli