Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged wild-type enzyme in mutant GlpT-deficient strain SH1200 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, rocker-switch mechanism of membrane transport by GlpT, overview | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | - |
Escherichia coli | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | GlpT is responsible for secondary active transport of substrate across the cell membrane, which is crucial to many cellular and physiological processes, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type enzyme from mutant GlpT-deficient strain SH1200 by ultracentrifugation and nickel affinity chromatography | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme, reconstitution of GlpT into proteoliposomes, overview | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate binding affinity assays | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | GlpT is responsible for secondary active transport of substrate across the cell membrane, which is crucial to many cellular and physiological processes, overview | Escherichia coli | ? | - |
? | |
additional information | mechanism for substrate translocation across the membrane that involves a rocker-switch-type movement of the protein, transport assays at different conditions using whole cells or purified enzyme, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G3P transporter | - |
Escherichia coli |
GlpT | - |
Escherichia coli |
sn-glycerol-3-phosphate transporter | - |
Escherichia coli |