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Literature summary for 7.6.2.10 extracted from

  • Law, C.J.; Yang, Q.; Soudant, C.; Maloney, P.C.; Wang, D.N.
    Kinetic evidence is consistent with the rocker-switch mechanism of membrane transport by GlpT (2007), Biochemistry, 46, 12190-12197.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.6.2.10

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged wild-type enzyme in mutant GlpT-deficient strain SH1200 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, rocker-switch mechanism of membrane transport by GlpT, overview Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli GlpT is responsible for secondary active transport of substrate across the cell membrane, which is crucial to many cellular and physiological processes, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type enzyme from mutant GlpT-deficient strain SH1200 by ultracentrifugation and nickel affinity chromatography Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
recombinant enzyme, reconstitution of GlpT into proteoliposomes, overview Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate binding affinity assays Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information GlpT is responsible for secondary active transport of substrate across the cell membrane, which is crucial to many cellular and physiological processes, overview Escherichia coli ?
-
 ?
additional information mechanism for substrate translocation across the membrane that involves a rocker-switch-type movement of the protein, transport assays at different conditions using whole cells or purified enzyme, overview Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
G3P transporter
-
 Escherichia coli
GlpT
-
 Escherichia coli
sn-glycerol-3-phosphate transporter
-
 Escherichia coli