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Literature summary for 7.6.2.1 extracted from

  • Vestergaard, A.L.; Mikkelsen, S.A.; Coleman, J.A.; Molday, R.S.; Vilsen, B.; Andersen, J.P.
    Specific mutations in mammalian P4-ATPase ATP8A2 catalytic subunit entail differential glycosylation of the accessory CDC50A subunit (2015), FEBS Lett., 589, 3908-3914 .
    View publication on PubMed
Search for more literature for 7.6.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells Bos taurus

Protein Variants

Protein Variants Comment Organism
E123A the mutant shows about 80% of wild type activity Bos taurus
E126A the mutant shows about 10% of wild type activity. The mutation leads to reduced glycosylation of the accessory subunit CDC50A Bos taurus
E198Q inactive Bos taurus
I305A the mutant shows about 50% of wild type activity. The mutation leads to reduced glycosylation of the accessory subunit CDC50A Bos taurus
I362A the mutant shows about 45% of wild type activity Bos taurus
L306A the mutant shows about 60% of wild type activity Bos taurus
L361A the mutant shows about 15% of wild type activity. The mutation leads to reduced glycosylation of the accessory subunit CDC50A Bos taurus
L366A the mutant shows about 40% of wild type activity. The mutation leads to reduced glycosylation of the accessory subunit CDC50A Bos taurus
L367A the mutant shows about 60% of wild type activity Bos taurus
L367F the mutant shows about 10% of wild type activity Bos taurus
L367P the mutant shows about 55% of wild type activity Bos taurus
L367Y the mutant shows about 40% of wild type activity Bos taurus
Y869A the mutant shows about 45% of wild type activity Bos taurus
Y878A the mutant shows about 25% of wild type activity. The mutation leads to reduced glycosylation of the accessory subunit CDC50A Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Bos taurus 5886
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose bead column chromatography Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + 1-oleoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-sn-glycero-3-phosphoserine[side 1]
-
 Bos taurus ADP + phosphate + 1-oleoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-sn-glycero-3-phosphoserine[side 2]
-
 ?

Synonyms

Synonyms Comment Organism
Atp8a2 catalytic subunit Bos taurus
CDC50A accessory subunit Bos taurus
flippase
-
 Bos taurus
P4-ATPase
-
 Bos taurus