Literature summary for 7.6.2.1 extracted from

Baldridge, R.D.; Graham, T.R.
Two-gate mechanism for phospholipid selection and transport by type IV P-type ATPases (2013), Proc. Natl. Acad. Sci. USA, 110, E358-E367.

Search for more literature for 7.6.2.1

Protein Variants

Protein Variants	Comment	Organism
I1235F	mutation increases the overall activity of Dnf1 for all substrates and causes partial loss of specificity for glycerophospholipid	Saccharomyces cerevisiae
additional information	generation of chimeras between isoforms Drs2 and Dnf1 by swapping of transmembrane domains. The mechanistic coupling of the entry and the exit gates seems to be conserved for Drs2. The residues that determine headgroup specificity for phospholipid transport cluster at two interfacial regions flanking transmembrane segments 1-4 and lie outside of the canonical substrate binding site operating in cation pumps. Two substrate-selecting gates act sequentially on opposite sides of the membrane: an entry gate, where phospholipid is initially selected from the extracellular leaflet, and an exit gate at the cytosolic leaflet. The entry and exit gates act cooperatively but imperfectly For example, the entry gate QQ to GA mutation causes a specific loss of phosphatidylserine asymmetry that can be restored by the N445S exit gate mutation	Saccharomyces cerevisiae
additional information	generation of chimeras between isoforms Drs2 and Dnf1 by swapping of transmembrane domains. Within the entry gate, residues specifically involved in the phosphatidylcholine preference of Dnf1 are Ile234, Phe235, Pro240, Gly241, Tyr575, Glu586, Phe587, Gly588, and Ile590, and involved in suppression of phosphatidylserine recognition are Gly230, Ala231, and K578. Within the exit gate, residues responsible for phosphatidylcholine preference are Ile545 and Glu622 and for suppression of phosphatidylserine transport are Tyr618, Asn550, Val621, and Glu622	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P32660	-	-
Saccharomyces cerevisiae	P39524	-	-

Synonyms

Synonyms	Comment	Organism
Dnf1	-	Saccharomyces cerevisiae
Drs2	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)