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Literature summary for 7.6.2.1 extracted from
- Lipid specific activation of the murine P4-ATPase Atp8a1 (ATPase II) (2006), Biochemistry, 45, 5367-5376.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the secretory granule Atp8a1 is activated by phospholipids binding to a specific site whose properties (PS selectivity, dependence upon glycerol but not serine, stereochemistry, and vanadate sensitivity) are similar to, but distinct from, the properties of the substrate binding site of the plasma membrane flippase, no activation by N-methylphosphatidylserine, phosphatidylserine-O-methyl ester, lysophosphatidylserine, glycerophosphoserine, and phosphoserine, Atp8a1 activities are insensitive to the stereochemistry of the serine headgroup of activating phospholipid | Mus musculus | |
| phosphatidylethanolamine | slight activation | Mus musculus |  |
| phosphatidylglycerol | slight activation | Mus musculus | |
| phosphatidylhomoserine | slight activation | Mus musculus | |
| phosphatidylhydroxypropionate | slight activation | Mus musculus | |
| phosphatidylserine | 2.1fold activation, Atp8a1 is activated only by the naturally occurring sn-1,2-glycerol isomer of PS and not the sn-2,3-glycerol stereoisomer | Mus musculus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged Atp8a1 in Spodoptera frugiperda Sf21 cells using the baculovirus transfection system | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Mus musculus | |
| sulfhydryl reagents | - |
Mus musculus | |
| vanadate | - |
Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Mus musculus | 5886 | - |
| secretory granule | - |
Mus musculus | 30141 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
x * 120000, recombinant His-tagged Atp8a1, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + phosphatidylserine/in | Mus musculus | selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the cell membrane to maintain lipid asymmetry in the plasma membrane and secretory vesicles | ADP + phosphate + phosphatidylserine/out | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged Atp8a1 from Spodoptera frugiperda Sf21 cells by microsome preparation and nickel affinity chromatography | Mus musculus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
Atp8a1 in Sf21 cell lysate, in absence of phosphatidylserine | Mus musculus |
| 4.9 | - |
Atp8a1 in Sf21 cell lysate, in presence of phosphatidylserine | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + phosphatidylserine/in | selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the cell membrane to maintain lipid asymmetry in the plasma membrane and secretory vesicles | Mus musculus | ADP + phosphate + phosphatidylserine/out | - |
? | |
| ATP + H2O + phosphatidylserine/in | selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the cell membrane to maintain lipid asymmetry | Mus musculus | ADP + phosphate + phosphatidylserine/out | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 120000, recombinant His-tagged Atp8a1, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminophospholipid flippase | - |
Mus musculus |
| ATP8A1 | - |
Mus musculus |
| More | the ATPase II belongs to the P4-ATPase family | Mus musculus |
| PS flippase | - |
Mus musculus |
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