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Literature summary for 7.6.2.1 extracted from

  • Vishwakarma, R.A.; Vehring, S.; Mehta, A.; Sinha, A.; Pomorski, T.; Herrmann, A.; Menon, A.K.
    New fluorescent probes reveal that flippase-mediated flip-flop of phosphatidylinositol across the endoplasmic reticulum membrane does not depend on the stereochemistry of the lipid (2005), Org. Biomol. Chem., 3, 1275-1283.
    View publication on PubMed
Search for more literature for 7.6.2.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information aminophospholipid translocase transbilayer movement stopped flow-kinetics in the reconstituted system or intact ER-vesicles with different fluorescence-labeled substrates Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
 Rattus norvegicus 5789
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus the enzyme is a key enzyme of membrane biogenesis performing transbilayer flipping of glycerophospholipids, e.g. in the endoplasmic reticulum, flipping is an ATP-independent, bidirectional process ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1D-myo-inositol 1-O-myristoyl-2-O-[6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino-hexanoyl]-sn-glycer-3-yl]-phosphate/in fluorescence-labeled substrate, proteoliposomes generated from flippase-enriched Triton X-100 extract of endoplasmic reticulum or native endoplasmic vesicles, overview Rattus norvegicus 1D-myo-inositol 1-O-myristoyl-2-O-[6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino-hexanoyl]-sn-glycer-3-yl]-phosphate/out
-
 ?
1D-myo-inositol 1-O-[1-[6'-([6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino]-hexanoyl]amino)hexanoyl]-2-O-stearoyl-sn-glycer-3-yl]-phosphate/in fluorescence-labeled substrate, proteoliposomes generated from flippase-enriched Triton X-100 extract of endoplasmic reticulum or native endoplasmic vesicles, overview Rattus norvegicus 1D-myo-inositol 1-O-[1-[6'-([6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino]-hexanoyl]amino)hexanoyl]-2-O-stearoyl-sn-glycer-3-yl]-phosphate/out
-
 ?
1D-myo-inositol 3-O-myristoyl-2-O-[6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino-hexanoyl]-sn-glycer-1-yl]-phosphate/in fluorescence-labeled substrate, proteoliposomes generated from flippase-enriched Triton X-100 extract of endoplasmic reticulum or native endoplasmic vesicles, overview Rattus norvegicus 1D-myo-inositol 3-O-myristoyl-2-O-[6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino-hexanoyl]-sn-glycer-1-yl]-phosphate/out
-
 ?
1D-myo-inositol 3-O-[1-[6'-([6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino]-hexanoyl]amino)hexanoyl]-2-O-stearoyl-sn-glycer-1-yl]-phosphate/in fluorescence-labeled substrate, proteoliposomes generated from flippase-enriched Triton X-100 extract of endoplasmic reticulum or native endoplasmic vesicles, overview Rattus norvegicus 1D-myo-inositol 3-O-[1-[6'-([6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino]-hexanoyl]amino)hexanoyl]-2-O-stearoyl-sn-glycer-1-yl]-phosphate/out
-
 ?
1L-myo-inositol 1-O-[1-[6'-([6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino]-hexanoyl]amino)hexanoyl]-2-O-stearoyl-sn-glycer-3-yl]-phosphate/in fluorescence-labeled substrate, proteoliposomes generated from flippase-enriched Triton X-100 extract of endoplasmic reticulum or native endoplasmic vesicles, overview Rattus norvegicus 1L-myo-inositol 1-O-[1-[6'-([6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino]-hexanoyl]amino)hexanoyl]-2-O-stearoyl-sn-glycer-3-yl]-phosphate/out
-
 ?
1L-myo-inositol 3-O-[1-[6'-([6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino]-hexanoyl]amino)hexanoyl]-2-O-stearoyl-sn-glycer-1-yl]-phosphate/in fluorescence-labeled substrate, proteoliposomes generated from flippase-enriched Triton X-100 extract of endoplasmic reticulum or native endoplasmic vesicles, overview Rattus norvegicus 1L-myo-inositol 3-O-[1-[6'-([6-[(7-nitro-2-oxa-1,3-diazolobenz-4-yl)amino]-hexanoyl]amino)hexanoyl]-2-O-stearoyl-sn-glycer-1-yl]-phosphate/out
-
 ?
additional information the enzyme is a key enzyme of membrane biogenesis performing transbilayer flipping of glycerophospholipids, e.g. in the endoplasmic reticulum, flipping is an ATP-independent, bidirectional process Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus