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Literature summary for 7.5.2.7 extracted from

  • Clifton, M.; Simon, M.; Erramilli, S.; Zhang, H.; Zaitseva, J.; Hermodson, M.; Stauffacher, C.
    In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes (2015), J. Biol. Chem., 290, 5555-5565 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.5.2.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
orthovanadate
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, maximal activity at 3 mM Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + D-ribose-[ribose-binding protein][side 1] Escherichia coli
-
 ADP + phosphate + D-ribose[side 2] + [ribose-binding protein][side 1]
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
a full RbsABC2 complex in the presence of stable, transition state mimics (ATP, Mg2+, and VO4), a RbsAC complex in the presence of ADP and Mg2+, and a RbsBC complex in the absence of cofactors are purified by Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + D-ribose-[ribose-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + D-ribose[side 2] + [ribose-binding protein][side 1]
-
 r

Synonyms

Synonyms Comment Organism
RbsABC2
-
 Escherichia coli
ribose ATP-binding cassette transporter
-
 Escherichia coli
ribose transporter the ribose transporter is a tripartite complex consisting of a cytoplasmic ATP-binding cassette protein, RbsA, with fused nucleotide binding domains, a transmembrane domain homodimer, RbsC2, and a periplasmic substrate binding protein, RbsB Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Escherichia coli
ATP
-
 Escherichia coli