Literature summary for 7.5.2.6 extracted from

Karow, M.; Georgopoulos, C.
The essential Escherichia coli msbA gene, a multicopy suppressor of null mutations in the htrB gene, is related to the universally conserved family of ATP-dependent translocators (1993), Mol. Microbiol., 7, 69-79 .

Search for more literature for 7.5.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
gene msbA, DNA and amino acid sequence determination and analysis, sequence comparisons, gene msbA may form an operon with a downstream gene orfE, complementation of the Escherichia coli htrB- phenotypes by expression of gene msbA from plasmid pK-Bst	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of an Escherichia coli msbA insertion knockout mutant. The mutation has a deleterious effect on bacterial growth or viability. The insertion mutation affects the expression of both genes, msbA and orfE, overview	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
inner membrane	an integral membrane protein	Escherichia coli	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + lipid A-core oligosaccharide[side 1]	Escherichia coli	-	ADP + phosphate + lipid A-core oligosaccharide[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P60752	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + lipid A-core oligosaccharide[side 1]	-	Escherichia coli	ADP + phosphate + lipid A-core oligosaccharide[side 2]	-	?

Subunits

Subunits	Comment	Organism
?	x * 64460, sequence calculation	Escherichia coli

Synonyms

Synonyms	Comment	Organism
MsbA	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	genes msbA and orfE, forming an operon, are essential for bacterial viability under all growth conditions tested. Gene msbA is a multicopy suppressor of gene htrB, overview. When cloned in vectors maintained at two to four copies per cell, the msbA gene complements most of the HtrB phenotypes, including the morphological alterations, the overproduction of phospholipids, and the lethality exhibited at non-permissive temperatures. Neither HtrB nor MsbB can fully substitute for MsbA function	Escherichia coli

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Release 2023.1 (January 2023)