Literature summary for 7.5.2.6 extracted from

Zhou, Z.; White, K.; Polissi, A.; Georgopoulos, C.; Raetz, C.
Function of Escherichia coli MsbA, an essential ABC family transporter, in lipid A and phospholipid biosynthesis (1998), J. Biol. Chem., 273, 12466-12475 .

Search for more literature for 7.5.2.6

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
inner membrane	-	Escherichia coli	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + lipid A-core oligosaccharide[side 1]	Escherichia coli	-	ADP + phosphate + lipid A-core oligosaccharide[side 2]	-	?
ATP + H2O + lipid A-core oligosaccharide[side 1]	Escherichia coli W3110	-	ADP + phosphate + lipid A-core oligosaccharide[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P60752	-	-
Escherichia coli W3110	P60752	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + lipid A-core oligosaccharide[side 1]	-	Escherichia coli	ADP + phosphate + lipid A-core oligosaccharide[side 2]	-	?
ATP + H2O + lipid A-core oligosaccharide[side 1]	-	Escherichia coli W3110	ADP + phosphate + lipid A-core oligosaccharide[side 2]	-	?

Synonyms

Synonyms	Comment	Organism
MsbA	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

General Information

General Information	Comment	Organism
malfunction	accumulation of lipid A species in the inner membranes of htrB- cells when msbA and orfE are not expressed. The tetra-acylated lipid A precursors that accumulate in htrB mutants may not be transported as efficiently by MsbA as are penta- or hexaacylated lipid A species. LPS accumulates in the inner membranes of htrB-deficient mutants. Introduction of msbA and orfE on low copy plasmids partially restores translocation of LPS to the outer membrane at 42°C in htrB mutants. The Escherichia coli msbA gene also functions as a multicopy suppressor of htrB mutations	Escherichia coli
physiological function	MsbA is an essential ABC family transporter in lipid A and phospholipid biosynthesis, ATP-dependent transport of nascent core-lipid A molecules across the inner membrane by MsbA. The Escherichia coli msbA gene also functions as a multicopy suppressor of htrB mutations (temperature-sensitive growth of an Escherichia coli mutant lacking htrB and its suppression by extra copies of msbA). The HtrB-catalyzed transfer of laurate to lipid A may be necessary for efficient core-lipid A transport and MsbA and/or OrfE are components of the transport machinery	Escherichia coli

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Release 2023.1 (January 2023)