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Literature summary for 7.5.2.6 extracted from

  • Doshi, R.; Ali, A.; Shi, W.; Freeman, E.V.; Fagg, L.A.; van Veen, H.W.
    Molecular disruption of the power stroke in the ATP-binding cassette transport protein MsbA (2013), J. Biol. Chem., 288, 6801-6813.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A281C the mutant displays a reduction in ATP-dependent Hoechst 33342 export activity Escherichia coli
E208A the mutant shows greatly reduced activity compared to the wild type enzyme Escherichia coli
E208A/A281C the mutant displays a strong reduction in ATP-dependent Hoechst 33342 export activity Escherichia coli
E208A/K212A the mutant shows greatly reduced activity compared to the wild type enzyme Escherichia coli
E208Q the mutant shows 44.4% of wild type activity Escherichia coli
K212A the mutant shows greatly reduced activity compared to the wild type enzyme Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
2 * 56000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P60752
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-nitrilotriacetic acid resin column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + ethidium/in
-
Escherichia coli ADP + phosphate + ethidium/out
-
?
ATP + H2O + Hoechst 33342/in
-
Escherichia coli ADP + phosphate + Hoechst 33342/out
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 56000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
MsbA
-
Escherichia coli