Protein Variants | Comment | Organism |
---|---|---|
A281C | the mutant displays a reduction in ATP-dependent Hoechst 33342 export activity | Escherichia coli |
E208A | the mutant shows greatly reduced activity compared to the wild type enzyme | Escherichia coli |
E208A/A281C | the mutant displays a strong reduction in ATP-dependent Hoechst 33342 export activity | Escherichia coli |
E208A/K212A | the mutant shows greatly reduced activity compared to the wild type enzyme | Escherichia coli |
E208Q | the mutant shows 44.4% of wild type activity | Escherichia coli |
K212A | the mutant shows greatly reduced activity compared to the wild type enzyme | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P60752 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-nitrilotriacetic acid resin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + ethidium/in | - |
Escherichia coli | ADP + phosphate + ethidium/out | - |
? | |
ATP + H2O + Hoechst 33342/in | - |
Escherichia coli | ADP + phosphate + Hoechst 33342/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 56000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
MsbA | - |
Escherichia coli |