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Literature summary for 7.5.2.6 extracted from

  • Schultz, K.; Merten, J.; Klug, C.
    Characterization of the E506Q and H537A dysfunctional mutants in the E. coli abc transporter MsbA (2011), Biochemistry, 50, 3599-3608.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
E506Q mutation leads to dysfunctional protein, loss of cell viability. Mutant protein maintains its ability to bind ATP, but hydrolysis is severely inhibited. Hydrolysis does occur over time. Protein adopts a closed dimer conformation, indicating that events within the cell can induce a stable, closed conformation of the MsbA homodimer that does not reopen even in the absence of nucleotide Escherichia coli
H537A mutation leads to dysfunctional protein, loss of cell viability. Mutant protein maintains its ability to bind ATP, but hydrolysis is severely inhibited. Hydrolysis does occur over time. Protein adopts a closed dimer conformation, indicating that events within the cell can induce a stable, closed conformation of the MsbA homodimer that does not reopen even in the absence of nucleotide Escherichia coli
S423C mutant shows a Vmax similar to WT Escherichia coli
S423C/E506Q significantly diminished rates of hydrolysis, about 4% of wild-type Escherichia coli
S423C/H537A significantly diminished rates of hydrolysis, about 4% of wild-type Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 ATP pH 7.0, 37°C, mutant H537A Escherichia coli
0.005
-
 ATP pH 7.0, 37°C, mutant S423C/E506Q Escherichia coli
0.005
-
 ATP pH 7.0, 37°C, mutant S423C/H537A Escherichia coli
0.012
-
 ATP pH 7.0, 37°C, mutant E506Q Escherichia coli
0.117
-
 ATP pH 7.0, 37°C, wild-type Escherichia coli
0.161
-
 ATP pH 7.0, 37°C, mutant S423C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Lipid A/in
-
 Escherichia coli ADP + phosphate + Lipid A/out
-
 ?

Synonyms

Synonyms Comment Organism
MsbA
-
 Escherichia coli