Literature summary for 7.5.2.6 extracted from

Zou, P.; McHaourab, H.S.
Alternating access of the putative substrate-binding chamber in the ABC transporter MsbA (2009), J. Mol. Biol., 393, 574-585.

Search for more literature for 7.5.2.6

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + H2O + lipopolysaccharide[side 1] = ADP + phosphate + lipopolysaccharide[side 2]	spin labeling and electron paramagnetic resonance spectroscopy are used to systematically explore rearrangements in MsbA structure during the ATP hydrolysis cycle. Spin-label accessibility and local dynamics are determined in liposomes for the nucleotide-free intermediate and the transition state of ATP hydrolysis. The changes in the electron paramagnetic resonance parameters between these two intermediates fit a global pattern consistent with alternating access of the chamber. In the transition state of ATP hydrolysis, spin labels on the cytoplasmic side report increase dynamic restrictions and reduce water accessibility, while those on the extracellular side report increase water penetration	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Lipid A/in	-	Escherichia coli	ADP + phosphate + Lipid A/out	-	?

Synonyms

Synonyms	Comment	Organism
MsbA	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Escherichia coli

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Release 2023.1 (January 2023)