Any feedback?
Literature summary for 7.5.2.6 extracted from
- Functional characterization of Escherichia coli MsbA: interaction with nucleotides and substrates (2008), J. Biol. Chem., 283, 12840-12850.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Lipid | from Escherichia coli, about 1.4fold stimulation | Escherichia coli |  |
| lipid A | about 2fold stimulation of the ATPase activity of MsbA by its substrate | Escherichia coli | |
| phosphocholine | 2fold stimulation of MsbA | Escherichia coli | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene msbA, overexpression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP-PCP | - |
Escherichia coli | |
| D-20133 | lipid-based drug, high affinity binding to MsbA | Escherichia coli | |
| ilmofosine | lipid-based drug, high affinity binding to MsbA | Escherichia coli | |
| leupeptin | - |
Escherichia coli | |
| lipopolysaccharide | dependent on the origin | Escherichia coli | |
| additional information | no inhibition by substrate lipid A also at high concentration, poor inhibition by verapamil, colchicine, and daunorubicine, poor inhibition by lipid-based drugs D-20133, and D-21266, along with LY335979 | Escherichia coli | |
| vanadate | - |
Escherichia coli | |
| vinblastin | - |
Escherichia coli | |
| vinblastine | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | lipid binding kinetics, overview | Escherichia coli | |
| 0.0064 | - |
lipid A | pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | |
| 0.048 | 0.05 | lipopolysaccharide | pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | |
| 2 | - |
ATP | pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| inner membrane | enzyme structure with 6 transmembrane helices, overview | Escherichia coli | - |
- |
| periplasm | - |
Escherichia coli | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Lipid A/in | Escherichia coli | - |
ADP + phosphate + Lipid A/out | - |
? | |
| additional information | Escherichia coli | MsbA functions as an ATP-dependent lipid translocase that transports lipid A from the inner to the outer leaflet of the cytoplasmic membrane. MsbA is able to hydrolyze TNP-ATP, albeit at a lower rate than its corresponding ATPase activity | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene msbA | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Lipid A/in | - |
Escherichia coli | ADP + phosphate + Lipid A/out | - |
? | |
| ATP + H2O + Lipid A/in | substrate binding structure, overview | Escherichia coli | ADP + phosphate + Lipid A/out | - |
? | |
| additional information | MsbA functions as an ATP-dependent lipid translocase that transports lipid A from the inner to the outer leaflet of the cytoplasmic membrane. MsbA is able to hydrolyze TNP-ATP, albeit at a lower rate than its corresponding ATPase activity | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | enzyme structure, modelling | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the ATP-binding cassette superfamily | Escherichia coli |
| MsbA | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Escherichia coli | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.48 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | leupeptin | |
| 0.5 | - |
above, pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | vinblastin | |
| 1.25 | - |
pH 7.5, 37°C, recombinant His-tagged protein | Escherichia coli | AMP-PCP | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
