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Literature summary for 7.5.2.6 extracted from
- Biochemical Characterization of MsbA from Pseudomonas aeruginosa (2007), J. Biol. Chem., 282, 26939-26947.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity shown to be selectively stimulated by different truncated versions of core oligosaccharides of lipopoysaccharides of Pseudomonas aeruginosa | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli, host strains BL21-DE3, JM109, SM10, different plasmids used and listed | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.495 | - |
ATP | 8 mM ATP, chimeric protein, reconstitution form | Pseudomonas aeruginosa |  |
| 0.573 | - |
ATP | 8 mM ATP, detergent solubilized form of protein | Pseudomonas aeruginosa | |
| 3.6 | - |
ATP | 8 mM ATP, chimeric protein, detergent solubilized form | Pseudomonas aeruginosa | |
| 4.5 | - |
ATP | 8 mM ATP, reconstitution form of protein | Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Pseudomonas aeruginosa | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + lipo-oligosaccharide/in | Pseudomonas aeruginosa | - |
ADP + phosphate + lipo-oligosaccharide/out | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9HUG8 | MsbA protein, encoded by pA4997; serotype O5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, gel filtration | Pseudomonas aeruginosa |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
kinetics of ATPase activity shown to be different from that of MsbA of Escherichia coli, colorimetric ATPase assay with purified and liposome-reconstituted protein performed, msbA mutants generated, antibiotic susceptibility test applied analyzing purified and reconstituted chimeric MsbA protein | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + lipo-oligosaccharide/in | - |
Pseudomonas aeruginosa | ADP + phosphate + lipo-oligosaccharide/out | - |
? | |
| ATP + H2O + lipo-oligosaccharide/in | MsbA protein shown as a member of the ABC transporter family, differences to MsbA protein of Escherichia coli determined | Pseudomonas aeruginosa | ADP + phosphate + lipo-oligosaccharide/out | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MsbA protein | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
ATPase assay at | Pseudomonas aeruginosa |
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