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Literature summary for 7.5.2.11 extracted from

  • Bulut, H.; Ma, Q.; Moniot, S.; Saenger, W.; Schneider, E.; Vahedi-Faridi, A.
    Crystal structures of receptors involved in small molecule transport across membranes (2012), Eur. J. Cell Biol., 91, 318-325 .
    View publication on PubMed
Search for more literature for 7.5.2.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 cells Actinoplanes sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting or hanging drop vapor diffusion method Actinoplanes sp.

Protein Variants

Protein Variants Comment Organism
D361E the mutant of subunit AcbH has no D-galactose binding ability Actinoplanes sp.
D361K the mutant of subunit AcbH has no D-galactose binding ability Actinoplanes sp.
R362A the mutant of subunit AcbH has no D-galactose binding ability Actinoplanes sp.
R362K the mutant of subunit AcbH has no D-galactose binding ability Actinoplanes sp.
R82A the mutant of subunit AcbH shows about 30% D-galactose binding activity with respect to the wild type Actinoplanes sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Actinoplanes sp. 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + D-galactose-[D-galactose-binding protein AcbH][side 1] Actinoplanes sp. the transporter is specific for D-galactose ADP + phosphate + D-galactose[side 2] + [D-galactose-binding protein AcbH][side 1]
-
 ?

Organism

Organism UniProt Comment Textmining
Actinoplanes sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Actinoplanes sp.

Renatured (Commentary)

Renatured (Comment) Organism
-
 Actinoplanes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + D-galactose-[D-galactose-binding protein AcbH][side 1] the transporter is specific for D-galactose Actinoplanes sp. ADP + phosphate + D-galactose[side 2] + [D-galactose-binding protein AcbH][side 1]
-
 ?
additional information no affinity for maltose or maltodextrins but rather low affinity for acarbose and its homologs, components 5C and 6AB Actinoplanes sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
AcbFGH
-
 Actinoplanes sp.