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Literature summary for 7.5.2.11 extracted from

  • Yadava, U.; Vetting, M.W.; Al Obaidi, N.; Carter, M.S.; Gerlt, J.A.; Almo, S.C.
    Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine (2016), Acta Crystallogr. Sect. F, 72, 467-472 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.5.2.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Agrobacterium vitis

Crystallization (Commentary)

Crystallization (Comment) Organism
substrate binding protein Avi_5305 bound to D-glucosamine and D-galactosamine, sitting drop vapor diffusion method, using 30-40% (w/v) PEG 4000 Agrobacterium vitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + D-galactosamine-[galactosamine-binding protein Avi_5305][side 1] Agrobacterium vitis
-
 ADP + phosphate + D-galactosamine[side 2] + [galactosamine-binding protein Avi_5305][side 1]
-
 ?
ATP + H2O + D-galactosamine-[galactosamine-binding protein Avi_5305][side 1] Agrobacterium vitis S4
-
 ADP + phosphate + D-galactosamine[side 2] + [galactosamine-binding protein Avi_5305][side 1]
-
 ?
ATP + H2O + D-glucosamine-[glucosamine-binding protein Avi_5305][side 1] Agrobacterium vitis
-
 ADP + phosphate + D-glucosamine[side 2] + [glucosamine-binding protein Avi_5305][side 1]
-
 ?
ATP + H2O + D-glucosamine-[glucosamine-binding protein Avi_5305][side 1] Agrobacterium vitis S4
-
 ADP + phosphate + D-glucosamine[side 2] + [glucosamine-binding protein Avi_5305][side 1]
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium vitis B9K0Q5
-
-
Agrobacterium vitis S4 B9K0Q5
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel resin column chromatography and Superdex S200 gel filtration Agrobacterium vitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + D-galactosamine-[galactosamine-binding protein Avi_5305][side 1]
-
 Agrobacterium vitis ADP + phosphate + D-galactosamine[side 2] + [galactosamine-binding protein Avi_5305][side 1]
-
 ?
ATP + H2O + D-galactosamine-[galactosamine-binding protein Avi_5305][side 1]
-
 Agrobacterium vitis S4 ADP + phosphate + D-galactosamine[side 2] + [galactosamine-binding protein Avi_5305][side 1]
-
 ?
ATP + H2O + D-glucosamine-[glucosamine-binding protein Avi_5305][side 1]
-
 Agrobacterium vitis ADP + phosphate + D-glucosamine[side 2] + [glucosamine-binding protein Avi_5305][side 1]
-
 ?
ATP + H2O + D-glucosamine-[glucosamine-binding protein Avi_5305][side 1]
-
 Agrobacterium vitis S4 ADP + phosphate + D-glucosamine[side 2] + [glucosamine-binding protein Avi_5305][side 1]
-
 ?
additional information the enzyme shows specificity for D-glucosamine/D-galactosamine over D-glucose/D-galactose Agrobacterium vitis ?
-
 ?
additional information the enzyme shows specificity for D-glucosamine/D-galactosamine over D-glucose/D-galactose Agrobacterium vitis S4 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 36800, calculated from amino acid sequence Agrobacterium vitis

Synonyms

Synonyms Comment Organism
Avi_5305 solute binding protein Agrobacterium vitis
Pfam13407
-
 Agrobacterium vitis