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Literature summary for 7.5.2.1 extracted from

  • Daus, M.L.; Grote, M.; Schneider, E.
    The MalF P2 loop of the ATP-binding cassette transporter MalFGK2 from Escherichia coli and Salmonella enterica serovar typhimurium interacts with maltose binding protein (MalE) throughout the catalytic cycle (2009), J. Bacteriol., 191, 754-761.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.5.2.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain JM109 Escherichia coli
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain JM109 Salmonella enterica

Protein Variants

Protein Variants Comment Organism
T31C/S205C site-directed mutagensis, formation of a crosslink betwwen the mutant subunits Escherichia coli
T31C/S205C site-directed mutagensis, formation of a crosslink betwwen the mutant subunits Salmonella enterica
T31C/T177C site-directed mutagenesis, the distance between MalF mutant and MalE T31C mutant changes from 10 A to 5 A upon binding of ATP or maltose, with a less pronounced result, and is reset to 10 A after hydrolysis of one ATP Escherichia coli
T31C/T177C site-directed mutagenesis, the distance between MalF mutant and MalE T31C mutant changes from 10 A to 5 A upon binding of ATP or maltose, with a less pronounced result, and is reset to 10 A after hydrolysis of one ATP Salmonella enterica
T80C/S205C site-directed mutagenesis, the mutation of subunit MalF mutant S205C does not affect the interaction with MalE mutant T80C Escherichia coli
T80C/S205C site-directed mutagenesis, the mutation of subunit MalF mutant S205C does not affect the interaction with MalE mutant T80C Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
vanadate traps the enzyme in a transition state-like conformation Escherichia coli
vanadate traps the enzyme in a transition state-like conformation Salmonella enterica

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-
membrane
-
 Salmonella enterica 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + maltose/out Escherichia coli
-
 ADP + phosphate + maltose/in
-
 ?
ATP + H2O + maltose/out Salmonella enterica
-
 ADP + phosphate + maltose/in
-
 ?
additional information Escherichia coli the MalF P2 loop plays an important role in intersubunit communication. In particular, this loop is involved in keeping MalE in close contact with the transporter. the MalF P2 loop of the ATP-binding cassette transporter MalFGK2 interacts with maltose binding protein MalE throughout the catalytic cycle, overview ?
-
 ?
additional information Salmonella enterica the MalF P2 loop plays an important role in intersubunit communication. In particular, this loop is involved in keeping MalE in close contact with the transporter. the MalF P2 loop of the ATP-binding cassette transporter MalFGK2 interacts with maltose binding protein MalE throughout the catalytic cycle, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Salmonella enterica
-
 serovar typhimurium
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by nickel affinity chromatography and ultrafiltration Escherichia coli
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by nickel affinity chromatography and ultrafiltration Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + maltose/out
-
 Escherichia coli ADP + phosphate + maltose/in
-
 ?
ATP + H2O + maltose/out
-
 Salmonella enterica ADP + phosphate + maltose/in
-
 ?
additional information the MalF P2 loop plays an important role in intersubunit communication. In particular, this loop is involved in keeping MalE in close contact with the transporter. the MalF P2 loop of the ATP-binding cassette transporter MalFGK2 interacts with maltose binding protein MalE throughout the catalytic cycle, overview Escherichia coli ?
-
 ?
additional information the MalF P2 loop plays an important role in intersubunit communication. In particular, this loop is involved in keeping MalE in close contact with the transporter. the MalF P2 loop of the ATP-binding cassette transporter MalFGK2 interacts with maltose binding protein MalE throughout the catalytic cycle, overview Salmonella enterica ?
-
 ?

Synonyms

Synonyms Comment Organism
MalFGK2
-
 Escherichia coli
MalFGK2
-
 Salmonella enterica
maltose ATP-binding cassette transporter
-
 Escherichia coli
maltose ATP-binding cassette transporter
-
 Salmonella enterica

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
ATP
-
 Salmonella enterica