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Literature summary for 7.4.2.9 extracted from

  • Kuenzl, T.; Li-Blatter, X.; Srivastava, P.; Herdewijn, P.; Sharpe, T.; Panke, S.
    Mutant variants of the substrate-binding protein DppA from Escherichia coli enhance growth on nonstandard gamma-glutamyl amide-containing peptides (2018), Appl. Environ. Microbiol., 84, e00340-18 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1451
-
 L-Ala-L-gamma-Glu-L-Leu-[L-Ala-L-gamma-Glu-L-Leu-binding protein][side 1] at pH 7.0 and 37°C Escherichia coli
1.335
-
 L-Ala-L-gamma-Glu-L-Phe-L-Leu-[L-Ala-L-gamma-Glu-L-Phe-L-Leu-binding protein][side 1] at pH 7.0 and 37°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a dipeptide-[dipeptide-binding protein][side 1] Escherichia coli
-
 ADP + phosphate + a dipeptide[side 2] + [dipeptide-binding protein][side 1]
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a dipeptide-[dipeptide-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + a dipeptide[side 2] + [dipeptide-binding protein][side 1]
-
 ?
ATP + H2O + L-Ala-L-gamma-Glu-L-Leu-[L-Ala-L-gamma-Glu-L-Leu-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + L-Ala-L-gamma-Glu-L-Leu[side 2] + [L-Ala-L-gamma-Glu-L-Leu-binding protein][side 1]
-
 ?
ATP + H2O + L-Ala-L-gamma-Glu-L-Phe-L-Leu-[L-Ala-L-gamma-Glu-L-Phe-L-Leu-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + L-Ala-L-gamma-Glu-L-Phe-L-Leu[side 2] + [L-Ala-L-gamma-Glu-L-Phe-L-Leu-binding protein][side 1]
-
 ?
ATP + H2O + L-Ala-L-gamma-Glu-O-phospho-L-homoserine-[L-Ala-L-gamma-Glu-O-phospho-L-homoserine-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + L-Ala-L-gamma-Glu-O-phospho-L-homoserine[side 2] + [L-Ala-L-gamma-Glu-O-phospho-L-homoserine-binding protein][side 1]
-
 ?
ATP + H2O + L-Ala-L-gamma-Glu-O-phospho-L-serine-[L-Ala-L-gamma-Glu-O-phospho-L-serine-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + L-Ala-L-gamma-Glu-O-phospho-L-serine[side 2] + [L-Ala-L-gamma-Glu-O-phospho-L-serine-binding protein][side 1]
-
 ?

Synonyms

Synonyms Comment Organism
dipeptide permease A
-
 Escherichia coli
DppA
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70.5
-
 melting temperature of unbound wild type enzyme Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.31
-
 L-Ala-L-gamma-Glu-L-Phe-L-Leu-[L-Ala-L-gamma-Glu-L-Phe-L-Leu-binding protein][side 1] at pH 7.0 and 37°C Escherichia coli
24.4
-
 L-Ala-L-gamma-Glu-L-Leu-[L-Ala-L-gamma-Glu-L-Leu-binding protein][side 1] at pH 7.0 and 37°C Escherichia coli

General Information

General Information Comment Organism
metabolism the enzyme is responsible for the initial binding of peptides in the periplasmic space Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.22
-
 L-Ala-L-gamma-Glu-L-Phe-L-Leu-[L-Ala-L-gamma-Glu-L-Phe-L-Leu-binding protein][side 1] at pH 7.0 and 37°C Escherichia coli
168
-
 L-Ala-L-gamma-Glu-L-Leu-[L-Ala-L-gamma-Glu-L-Leu-binding protein][side 1] at pH 7.0 and 37°C Escherichia coli